dc.contributor.author
Vergara-Barros, Pablo
dc.contributor.author
Alcorta, Jaime
dc.contributor.author
Casanova-Katny, Angélica
dc.contributor.author
Nürnberg, Dennis J.
dc.contributor.author
Díez, Beatriz
dc.date.accessioned
2023-01-06T13:34:25Z
dc.date.available
2023-01-06T13:34:25Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/37487
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-37201
dc.description.abstract
Key organisms in the environment, such as oxygenic photosynthetic primary producers (photosynthetic eukaryotes and cyanobacteria), are responsible for fixing most of the carbon globally. However, they are affected by environmental conditions, such as temperature, which in turn affect their distribution. Globally, the cyanobacterium Fischerella thermalis is one of the main primary producers in terrestrial hot springs with thermal gradients up to 60 °C, but the mechanisms by which F. thermalis maintains its photosynthetic activity at these high temperatures are not known. In this study, we used molecular approaches and bioinformatics, in addition to photophysiological analyses, to determine the genetic activity associated with the energy metabolism of F. thermalis both in situ and in high-temperature (40 °C to 65 °C) cultures. Our results show that photosynthesis of F. thermalis decays with temperature, while increased transcriptional activity of genes encoding photosystem II reaction center proteins, such as PsbA (D1), could help overcome thermal damage at up to 60 °C. We observed that F. thermalis tends to lose copies of the standard G4 D1 isoform while maintaining the recently described D1INT isoform, suggesting a preference for photoresistant isoforms in response to the thermal gradient. The transcriptional activity and metabolic characteristics of F. thermalis, as measured by metatranscriptomics, further suggest that carbon metabolism occurs in parallel with photosynthesis, thereby assisting in energy acquisition under high temperatures at which other photosynthetic organisms cannot survive. This study reveals that, to cope with the harsh conditions of hot springs, F. thermalis has several compensatory adaptations, and provides emerging evidence for mixotrophic metabolism as being potentially relevant to the thermotolerance of this species. Ultimately, this work increases our knowledge about thermal adaptation strategies of cyanobacteria.
en
dc.format.extent
19 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
thermophiles
en
dc.subject
cyanobacteria
en
dc.subject
Fischerella thermalis
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::550 Geowissenschaften, Geologie::551 Geologie, Hydrologie, Meteorologie
dc.subject.ddc
500 Naturwissenschaften und Mathematik::530 Physik::530 Physik
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie
dc.title
Compensatory Transcriptional Response of Fischerella thermalis to Thermal Damage of the Photosynthetic Electron Transfer Chain
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
8515
dcterms.bibliographicCitation.doi
10.3390/molecules27238515
dcterms.bibliographicCitation.journaltitle
Molecules
dcterms.bibliographicCitation.number
23
dcterms.bibliographicCitation.originalpublishername
MDPI
dcterms.bibliographicCitation.volume
27
dcterms.bibliographicCitation.url
https://doi.org/10.3390/molecules27238515
refubium.affiliation
Physik
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1420-3049