dc.contributor.author
Witt, Natalie
dc.contributor.author
Galante, Domenico
dc.contributor.author
Andreotti, Sandro
dc.contributor.author
Abdel Glil, Mostafa
dc.contributor.author
Fasanella, Antonio
dc.contributor.author
Meierhofer, David
dc.contributor.author
Tomaso, Herbert
dc.date.accessioned
2022-12-30T12:21:33Z
dc.date.available
2022-12-30T12:21:33Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/37371
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-37083
dc.description.abstract
Anthrax is a zoonotic infection caused by the bacterium Bacillus anthracis (BA). Specific identification of this pathogen often relies on targeting genes located on two extrachromosomal plasmids, which represent the major pathogenicity factors of BA. However, more recent findings show that these plasmids have also been found in other closely related Bacillus species. In this study, we investigated the possibility of identifying species-specific and universally applicable marker peptides for BA. For this purpose, we applied a high-resolution mass spectrometry-based approach for 42 BA isolates. Along with the genomic sequencing data and by developing a bioinformatics data evaluation pipeline, which uses a database containing most of the publicly available protein sequences worldwide (UniParc), we were able to identify eleven universal marker peptides unique to BA. These markers are located on the chromosome and therefore, might overcome known problems, such as observable loss of plasmids in environmental species, plasmid loss during cultivation in the lab, and the fact that the virulence plasmids are not necessarily a unique feature of BA. The identified chromosomally encoded markers in this study could extend the small panel of already existing chromosomal targets and along with targets for the virulence plasmids, may pave the way to an even more reliable identification of BA using genomics- as well as proteomics-based techniques.
en
dc.format.extent
12 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Bacillus anthracis
en
dc.subject
high-resolutionmass spectrometry
en
dc.subject.ddc
000 Informatik, Informationswissenschaft, allgemeine Werke::000 Informatik, Wissen, Systeme::004 Datenverarbeitung; Informatik
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie
dc.title
Identification of Universally Applicable and Species-Specific Marker Peptides for Bacillus anthracis
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
1549
dcterms.bibliographicCitation.doi
10.3390/life12101549
dcterms.bibliographicCitation.journaltitle
Life
dcterms.bibliographicCitation.number
10
dcterms.bibliographicCitation.originalpublishername
MDPI
dcterms.bibliographicCitation.volume
12
dcterms.bibliographicCitation.url
https://doi.org/10.3390/life12101549
refubium.affiliation
Mathematik und Informatik
refubium.affiliation.other
Institut für Informatik
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
2075-1729