dc.contributor.author
Jo, Wendy K.
dc.contributor.author
Alfonso‐Toledo, Jorge A.
dc.contributor.author
Salas‐Rojas, Monica
dc.contributor.author
Almazan‐Marin, Cenia
dc.contributor.author
Galvez‐Romero, Guillermo
dc.contributor.author
García‐Baltazar, Anahí
dc.contributor.author
Obregón‐Morales, Cirani
dc.contributor.author
Rendón‐Franco, Emilio
dc.contributor.author
Kühne, Arne
dc.contributor.author
Carvalho‐Urbieta, Victor
dc.contributor.author
Rasche, Andrea
dc.contributor.author
Brünink, Sebastian
dc.contributor.author
Glebe, Dieter
dc.contributor.author
Aguilar‐Setién, Álvaro
dc.contributor.author
Drexler, Jan Felix
dc.date.accessioned
2022-12-02T12:42:20Z
dc.date.available
2022-12-02T12:42:20Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/37144
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-36857
dc.description.abstract
In humans, co-infection of hepatitis B and C viruses (HBV, HCV) is common and aggravates disease outcome. Infection-mediated disease aggravation is poorly understood, partly due to lack of suitable animal models. Carnivores are understudied for hepatitis virus homologues. We investigated Mexican carnivores (ringtails, Bassariscus astutus) for HBV and HCV homologues. Three out of eight animals were infected with a divergent HBV termed ringtail HBV (RtHBV) at high viral loads of 5 x 10(9) -1.4 x 10(10) copies/ml serum. Two of the RtHBV-infected animals were co-infected with a divergent hepacivirus termed ringtail hepacivirus (RtHV) at 4 x 10(6)-7.5 x 10(7) copies/ml in strain-specific qRT-PCR assays. Immunofluorescence assays relying on HBV core and RtHV NS3/4a proteins indicated that none of the animals had detectable hepadnavirus core-specific antibodies, whereas one RtHV-infected animal had concomitant RtHV-specific antibodies at 1:800 end-point titre. RtHBV and RtHV complete genomes showed typical HBV and HCV structure and length. All RtHBV genomes were identical, whereas RtHV genomes showed four amino acid substitutions located predominantly in the E1/E2-encoding genomic regions. Both RtHBV (>28% genomic nucleotide sequence distance) and RtHV (>30% partial NS3/NS5B amino acid sequence distance) formed new species within their virus families. Evolutionary analyses showed that RtHBV grouped with HBV homologues from different laurasiatherian hosts (carnivores, bats, and ungulates), whereas RtHV grouped predominantly with rodent-borne viruses. Ancestral state reconstructions showed that RtHV, but not RtHBV, likely emerged via a non-recent host switch involving rodent-borne hepacivirus ancestors. Conserved hepatitis virus infection patterns in naturally infected ringtails indicate that carnivores may be promising animal models to understand HBV/HCV co-infection.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
co-infection
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Natural co‐infection of divergent hepatitis B and C virus homologues in carnivores
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.doi
10.1111/tbed.14340
dcterms.bibliographicCitation.journaltitle
Transboundary and Emerging Diseases
dcterms.bibliographicCitation.number
2
dcterms.bibliographicCitation.originalpublishername
Wiley
dcterms.bibliographicCitation.pagestart
195
dcterms.bibliographicCitation.pageend
203
dcterms.bibliographicCitation.volume
69
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.funding
DEAL Wiley
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
34606685
dcterms.isPartOf.issn
1865-1674
dcterms.isPartOf.eissn
1865-1682