dc.contributor.author
Reichelt, Benjamin
dc.contributor.author
Szott, Vanessa
dc.contributor.author
Epping, Lennard
dc.contributor.author
Semmler, Torsten
dc.contributor.author
Merle, Roswitha
dc.contributor.author
Roesler, Uwe
dc.contributor.author
Friese, Anika
dc.date.accessioned
2022-11-01T10:44:52Z
dc.date.available
2022-11-01T10:44:52Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/36665
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-36378
dc.description.abstract
Broiler meat is widely known as an important source of foodborne Campylobacter jejuni and Campylobacter coli infections in humans. In this study, we thoroughly investigated transmission pathways that may contribute to possible Campylobacter contamination inside and outside broiler houses. For this purpose we carried out a comprehensive longitudinal sampling approach, using a semi-quantitative cultivation method to identify and quantify transmissions and reservoirs of Campylobacter spp.. Three german broiler farms in Brandenburg and their surrounding areas were intensively sampled, from April 2018 until September 2020. Consecutive fattening cycles and intervening downtimes after cleaning and disinfection were systematically sampled in summer and winter. To display the potential phylogeny of barn and environmental isolates, whole genome sequencing (WGS) and bioinformatic analyses were performed. Results obtained in this study showed very high Campylobacter prevalence in 51/76 pooled feces (67.1%) and 49/76 boot swabs (64.5%). Average counts between 6.4 to 8.36 log10MPN/g were detected in pooled feces. In addition, levels of 4.7 and 4.1 log10MPN/g were detected in boot swabs and litter, respectively. Samples from the barn interior showed mean Campyloacter values in swabs from drinkers 2.6 log10MPN/g, walls 2.0 log10MPN/g, troughs 1.7 log10MPN/g, boards 1.6 log10MPN/g, ventilations 0.9 log10MPN/g and 0.7 log10MPN/g for air samples. However, Campylobacter was detected only in 7/456 (1.5%) of the environmental samples (water bodies, puddles or water-filled wheel tracks; average of 0.6 log10MPN/g). Furthermore, WGS showed recurring Campylobacter genotypes over several consecutive fattening periods, indicating that Campylobacter genotypes persist in the environment during downtime periods. However, after cleaning and disinfection of the barns, we were unable to identify potential sources in the broiler houses. Interestingly, alternating Campylobacter genotypes were observed after each fattening period, also indicating sources of contamination from the wider environment outside the farm. Therefore, the results of this study suggest that a potential risk of Campylobacter transmission may originate from present environmental sources (litter and water reservoirs). However, the sources of Campylobacter transmission may vary depending on the operation and farm environmental conditions.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
campylobacter
en
dc.subject
transmission
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
dc.title
Transmission pathways of campylobacter spp. at broiler farms and their environment in Brandenburg, Germany
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
982693
dcterms.bibliographicCitation.doi
10.3389/fmicb.2022.982693
dcterms.bibliographicCitation.journaltitle
Frontiers in Microbiology
dcterms.bibliographicCitation.volume
13 (2022)
dcterms.bibliographicCitation.url
https://doi.org/10.3389/fmicb.2022.982693
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Tier- und Umwelthygiene
refubium.note.author
Open Access Funding provided by the Freie Universität Berlin.
en
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1664-302X