dc.contributor.author
Giacomello, Ginevra
dc.contributor.author
Böttcher, Chotima
dc.contributor.author
Parr, Maria Kristina
dc.date.accessioned
2022-10-13T08:10:56Z
dc.date.available
2022-10-13T08:10:56Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/36557
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-36270
dc.description.abstract
Differences in metabolic profiles can link to functional changes of immune cells in disease conditions. Here, we detail a protocol for the detection and quantitation of 19 metabolites in one analytical run. We provide the parameters for chromatographic separation and mass spectrometric analysis of isotopically labeled and unlabeled metabolites. We include steps for incubation and sample preparation of PBMCs and monocytes. This protocol overcomes the chromatographic challenges caused by the chelating properties of some metabolites.
en
dc.format.extent
25 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Mass spectrometry
en
dc.subject
Metabolomics
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::615 Pharmakologie, Therapeutik
dc.title
Isotopic tracing of glucose metabolites in human monocytes to assess changes in inflammatory conditions
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
101715
dcterms.bibliographicCitation.doi
10.1016/j.xpro.2022.101715
dcterms.bibliographicCitation.journaltitle
STAR Protocols
dcterms.bibliographicCitation.number
4
dcterms.bibliographicCitation.volume
3 (2022)
dcterms.bibliographicCitation.url
https://doi.org/10.1016/j.xpro.2022.101715
refubium.affiliation
Biologie, Chemie, Pharmazie
refubium.affiliation.other
Institut für Pharmazie
refubium.funding
Publikationsfonds FU
refubium.note.author
Die Publikation wurde aus Open Access Publikationsgeldern der Freien Universität Berlin gefördert.
en
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
2666-1667
