dc.contributor.author
Filor, Viviane
dc.contributor.author
Seeger, Bettina
dc.contributor.author
Buhr, Nicole de
dc.contributor.author
Köckritz-Blickwede, Maren von
dc.contributor.author
Kietzmann, Manfred
dc.contributor.author
Oltmanns, Hilke
dc.contributor.author
Meißner, Jessica
dc.date.accessioned
2022-10-06T13:25:41Z
dc.date.available
2022-10-06T13:25:41Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/36499
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-36212
dc.description.abstract
Mastitis in cattle is a major health problem as well as incurring high costs for the dairy industry. To assess the suitability of precision-cut bovine udder slices (PCBUS) for bovine mastitis studies, we infected PCBUS with 2 different Staphylococcus aureus strains. Accordingly, we investigated both the tissue response to infection based on immune mediators at the mRNA and protein levels and the invasion of bacteria within the tissue. The studied proteins represent immune mediators of early inflammation [IL-1β, tumor necrosis factor-α (TNF-α), prostaglandin E2 (PGE2)] and showed a time-dependent increase in concentration. Infection of PCBUS with S. aureus resulted in increased expression of proinflammatory cytokines and chemokines such as TNF-α, C-C motif chemokine ligand 20 (CCL20), IL-1β, IL-6, and IL-10, but not C-X-C motif chemokine ligand 8 (CXCL8), lingual antimicrobial peptide (LAP), or S100 calcium binding protein A9 (S100A9) at the mRNA level. To compare the data acquired with this model, we carried out investigations on primary bovine mammary epithelial cells. Our results showed that the immune responses of both models - PCBUS and primary bovine mammary epithelial cells - were similar. In addition, investigations using PCBUS enabled us to demonstrate adherence of bacteria in the physiological cell network. These findings support the use of PCBUS in studies designed to further understand the complex pathophysiological processes of infection and inflammation in bovine mastitis and to investigate alternative therapies for mastitis.
en
dc.format.extent
14 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
bovine mastitis
en
dc.subject
precision-cut bovine udder slices
en
dc.subject
primary bovine mammary epithelial cells
en
dc.subject
Staphylococcus aureus
en
dc.subject
immune response
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::636 Viehwirtschaft
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::616 Krankheiten
dc.title
Investigation of the pathophysiology of bacterial mastitis using precision-cut bovine udder slices
dc.type
Wissenschaftlicher Artikel
dc.identifier.sepid
89026
dcterms.bibliographicCitation.doi
10.3168/jds.2021-21533
dcterms.bibliographicCitation.journaltitle
Journal of Dairy Science
dcterms.bibliographicCitation.number
9
dcterms.bibliographicCitation.originalpublishername
Elsevier
dcterms.bibliographicCitation.pagestart
7705
dcterms.bibliographicCitation.pageend
7718
dcterms.bibliographicCitation.volume
105
dcterms.bibliographicCitation.url
https://doi.org/10.3168/jds.2021-21533
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Pharmakologie und Toxikologie

refubium.funding
Publikationsfonds FU
refubium.note.author
Die Publikation wurde aus Open Access Publikationsgeldern der Freien Universität Berlin gefördert.
de
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1525-3198