dc.contributor.author
Vester, Karen
dc.contributor.author
Preußner, Marco
dc.contributor.author
Holton, Nicole
dc.contributor.author
Feng, Suihan
dc.contributor.author
Schultz, Carsten
dc.contributor.author
Heyd, Florian
dc.contributor.author
Wahl, Markus C.
dc.date.accessioned
2022-09-02T09:53:49Z
dc.date.available
2022-09-02T09:53:49Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/36125
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-35841
dc.description.abstract
Precursor messenger RNA splicing is a highly regulated process, mediated by a complex RNA-protein machinery, the spliceosome, that encompasses several hundred proteins and five small nuclear RNAs in humans. Emerging evidence suggests that the spatial organization of splicing factors and their spatio-temporal dynamics participate in the regulation of splicing. So far, methods to manipulate the spatial distribution of splicing factors in a temporally defined manner in living cells are missing. Here, we describe such an approach that takes advantage of a reversible chemical dimerizer, and outline the requirements for efficient, reversible re-localization of splicing factors to selected sub-nuclear compartments. In a proof-of-principle study, the partial re-localization of the PRPF38A protein to the nuclear lamina in HEK293T cells induced a moderate increase in intron retention. Our approach allows fast and reversible re-localization of splicing factors, has few side effects and can be applied to many splicing factors by fusion of a protein tag through genome engineering. Apart from the systematic analysis of the spatio-temporal aspects of splicing regulation, the approach has a large potential for the fast induction and reversal of splicing switches and can reveal mechanisms of splicing regulation in native nuclear environments.
en
dc.format.extent
14 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
RNA splicing
en
dc.subject
splicing factor
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie
dc.title
Recruitment of a splicing factor to the nuclear lamina for its inactivation
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
736
dcterms.bibliographicCitation.doi
10.1038/s42003-022-03689-y
dcterms.bibliographicCitation.journaltitle
Communications Biology
dcterms.bibliographicCitation.number
1
dcterms.bibliographicCitation.volume
5
dcterms.bibliographicCitation.url
https://doi.org/10.1038/s42003-022-03689-y
refubium.affiliation
Biologie, Chemie, Pharmazie
refubium.affiliation.other
Institut für Chemie und Biochemie
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refubium.funding
Springer Nature DEAL
refubium.note.author
Die Publikation wurde aus Open Access Publikationsgeldern der Freien Universität Berlin gefördert.
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
2399-3642
refubium.resourceType.provider
WoS-Alert