dc.contributor.author
Tyrikos-Ergas, Theodore
dc.contributor.author
Gim, Soeun
dc.contributor.author
Huang, Jhih-Yi
dc.contributor.author
Martin, Sandra Pinzon
dc.contributor.author
Silva, Daniel Varon
dc.contributor.author
Seeberger, Peter H.
dc.contributor.author
Delbianco, Martina
dc.date.accessioned
2022-08-31T11:18:16Z
dc.date.available
2022-08-31T11:18:16Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/36097
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-35813
dc.description.abstract
Bacterial biofilm matrices are nanocomposites of proteins and polysaccharides with remarkable mechanical properties. Efforts understanding and tuning the protein component have been extensive, whereas the polysaccharide part remained mostly overlooked. The discovery of phosphoethanolamine (pEtN) modified cellulose in E. coli biofilms revealed that polysaccharide functionalization alters the biofilm properties. To date, the pattern of pEtN cellulose and its mode of interactions with proteins remains elusive. Herein, we report a model system based on synthetic epitomes to explore the role of pEtN in biofilm-inspired assemblies. Nine pEtN-modified oligosaccharides were synthesized with full control over the length, degree and pattern of pEtN substitution. The oligomers were co-assembled with a representative peptide, triggering the formation of fibers in a length dependent manner. We discovered that the pEtN pattern modulates the adhesion of biofilm-inspired matrices, while the peptide component controls its stiffness. Unnatural oligosaccharides tune or disrupt the assembly morphology, revealing interesting targets for polysaccharide engineering to develop tunable bio-inspired materials.
en
dc.format.extent
8 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Bioinspired materials
en
dc.subject
Carbohydrate chemistry
en
dc.subject
Polysaccharides
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::540 Chemie::540 Chemie und zugeordnete Wissenschaften
dc.title
Synthetic phosphoethanolamine-modified oligosaccharides reveal the importance of glycan length and substitution in biofilm-inspired assemblies
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
3954
dcterms.bibliographicCitation.doi
10.1038/s41467-022-31633-5
dcterms.bibliographicCitation.journaltitle
Nature Communications
dcterms.bibliographicCitation.number
1
dcterms.bibliographicCitation.volume
13
dcterms.bibliographicCitation.url
https://doi.org/10.1038/s41467-022-31633-5
refubium.affiliation
Biologie, Chemie, Pharmazie
refubium.affiliation.other
Institut für Chemie und Biochemie
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refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
2041-1723
refubium.resourceType.provider
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