dc.contributor.author
Brunner, Nora
dc.contributor.author
Stein, Laura
dc.contributor.author
Amasheh, Salah
dc.date.accessioned
2023-02-02T08:37:02Z
dc.date.available
2023-02-02T08:37:02Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/35747
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-35462
dc.description.abstract
Epithelial barriers constitute a fundamental requirement in every organism, as they allow the separation of different environments and set boundaries against noxious and other adverse effectors. In many inflammatory and degenerative diseases, epithelial barrier function is impaired because of a disturbance of the paracellular seal. Recently, the Xenopus laevis oocyte has been established as a heterologous expression model for the analysis of transmembrane tight junction protein interactions and is currently considered to be a suitable screening model for barrier effectors. A prerequisite for this application is a physiological anchoring of claudins to the cytoskeleton via the major scaffolding protein tjp1 (tight junction protein 1, ZO-1). We have analyzed the oocyte model with regard to the interaction of heterologously expressed claudins and tjp1. Our experiments have revealed endogenous tjp1 expression in protein and mRNA analyses of unfertilized Xenopus laevis oocytes expressing human claudin 1 (CLDN1) to claudin 5 (CLDN5). The amphibian cell model can therefore be used for the analysis of claudin interactions.
en
dc.format.extent
11 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Zonula occludens 1
en
dc.subject
Xenopus oocytes
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
dc.title
Cellular Distribution Pattern of tjp1 (ZO-1) in Xenopus laevis Oocytes Heterologously Expressing Claudins
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.doi
10.1007/s00232-022-00251-z
dcterms.bibliographicCitation.journaltitle
The Journal of Membrane Biology
dcterms.bibliographicCitation.number
1
dcterms.bibliographicCitation.pagestart
51
dcterms.bibliographicCitation.pageend
61
dcterms.bibliographicCitation.volume
256
dcterms.bibliographicCitation.url
https://doi.org/10.1007/s00232-022-00251-z
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Veterinär-Physiologie
refubium.funding
Springer Nature DEAL
refubium.note.author
Die Publikation wurde aus Open Access Publikationsgeldern der Freien Universität Berlin gefördert.
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1432-1424
refubium.resourceType.provider
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