dc.contributor.author
Kanevche, Katerina
dc.date.accessioned
2022-04-08T09:19:22Z
dc.date.available
2022-04-08T09:19:22Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/34584
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-34302
dc.description.abstract
Infrared (IR) microscopy and spectroscopy, while being sensitive to the samples’ chemical composition, suffer from poor lateral resolution due to diffraction. Therefore, imaging of the cellular inner structure in the IR range of the electromagnetic spectrum proves to be challenging. An approach to overcome this limitation is by using scattering-type near-field optical microscopy (sSNOM) and nano-Fourier transform infrared (nanoFTIR) spectroscopy, achieved by combining the high spatial resolution of atomic force microscopy (AFM) and the chemical sensitivity of IR absorption. This methodology was applied to various biophysical systems with increasing complexity, starting with native membrane proteins, followed by aggregating peptides and light-induced surface patterning, concluding with investigation of cells.
In this work I was able to resolve the subcellular structure of C. reinhardtii and assign the IR absorption of various organelles to molecular vibrations with spatial resolution of 20 nm. The necessity and power of chemical imaging was demonstrated by scanning the nuclear area, where several nuclear bodies were distinguished in the sSNOM images while remaining hidden in the AFM topography. Finally, a stack of sSNOM images, obtained by sequential scanning of serial sections, was used to reconstruct a three-dimensional image. Thus, we demonstrate that sSNOM tomography allows visualizing three-dimensional intracellular structures at nanometer resolution where the contrast
originates from molecular vibrations of chemical bonds.
en
dc.format.extent
xxiii, 133 Seiten
dc.rights.uri
http://www.fu-berlin.de/sites/refubium/rechtliches/Nutzungsbedingungen
dc.subject
sSNOM microscopy
en
dc.subject
nanoFTIR spectroscopy
en
dc.subject
subcellular midIR imaging
en
dc.subject.ddc
500 Natural sciences and mathematics::530 Physics::530 Physics
dc.title
IR Near-field Nanoscopy and Spectroscopy of Cells and Soft Matter
dc.contributor.gender
female
dc.contributor.firstReferee
Heberle, Joachim
dc.contributor.furtherReferee
Kiril, Bolotin
dc.date.accepted
2022-03-21
dc.identifier.urn
urn:nbn:de:kobv:188-refubium-34584-9
refubium.affiliation
Physik
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open access
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accept