dc.contributor.author
Barcena, Maria Luisa
dc.contributor.author
Niehues, Maximilian H.
dc.contributor.author
Christiansen, Céline
dc.contributor.author
Estepa, Misael
dc.contributor.author
Haritonow, Natalie
dc.contributor.author
Sadighi, Amir H.
dc.contributor.author
Müller-Werdan, Ursula
dc.contributor.author
Ladilov, Yury
dc.contributor.author
Regitz-Zagrosek, Vera
dc.date.accessioned
2022-03-22T09:37:28Z
dc.date.available
2022-03-22T09:37:28Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/34457
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-34175
dc.description.abstract
Mounting evidence argues for the significant impact of sex in numerous cardiac pathologies, including myocarditis. Macrophage polarization and activation of cardiac fibroblasts play a key role in myocardial inflammation and remodeling. However, the role of sex in these processes is still poorly understood. In this study, we investigated sex-specific alterations in the polarization of murine bone marrow-derived macrophages (BMMs) and the polarization-related changes in fibroblast activation. Cultured male and female murine BMMs from C57/BL6J mice were polarized into M1 (LPS) and M2 (IL-4/IL-13) macrophages. Furthermore, male and female cardiac fibroblasts from C57/BL6J mice were activated with TNF-α, TGF-β, or conditioned medium from M1 BMMs. We found a significant overexpression of M1 markers (c-fos, NFκB, TNF-α, and IL-1β) and M2 markers (MCP-1 and YM1) in male but not female activated macrophages. In addition, the ROS levels were higher in M1 male BMMs, indicating a stronger polarization. Similarly, the pro-fibrotic markers TGF-β and IL-1β were expressed in activated cardiac male fibroblasts at a significantly higher level than in female fibroblasts. In conclusion, the present study provides strong evidence for the male-specific polarization of BMMs and activation of cardiac fibroblasts in an inflammatory environment. The data show an increased inflammatory response and tissue remodeling in male mice.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
sex differences
en
dc.subject
inflammation
en
dc.subject
bone marrow macrophages
en
dc.subject
macrophage phenotype
en
dc.subject
activated fibroblasts
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Male Macrophages and Fibroblasts from C57/BL6J Mice Are More Susceptible to Inflammatory Stimuli
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
758767
dcterms.bibliographicCitation.doi
10.3389/fimmu.2021.758767
dcterms.bibliographicCitation.journaltitle
Frontiers in Immunology
dcterms.bibliographicCitation.originalpublishername
Frontiers Media SA
dcterms.bibliographicCitation.volume
12
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
34867999
dcterms.isPartOf.eissn
1664-3224