dc.contributor.author
Blottner, Dieter
dc.contributor.author
Trautmann, Gabor
dc.contributor.author
Furlan, Sandra
dc.contributor.author
Gambara, Guido
dc.contributor.author
Block, Katharina
dc.contributor.author
Gutsmann, Martina
dc.contributor.author
Sun, Lian-Wen
dc.contributor.author
Worley, Paul F.
dc.contributor.author
Gorza, Luisa
dc.contributor.author
Scano, Martina
dc.contributor.author
Lorenzon, Paola
dc.contributor.author
Vida, Imre
dc.contributor.author
Volpe, Pompeo
dc.contributor.author
Salanova, Michele
dc.date.accessioned
2022-03-22T09:06:11Z
dc.date.available
2022-03-22T09:06:11Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/34455
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-34173
dc.description.abstract
The molecular mechanisms of skeletal muscle atrophy under extended periods of either disuse or microgravity are not yet fully understood. The transition of Homer isoforms may play a key role during neuromuscular junction (NMJ) imbalance/plasticity in space. Here, we investigated the expression pattern of Homer short and long isoforms by gene array, qPCR, biochemistry, and laser confocal microscopy in skeletal muscles from male C57Bl/N6 mice (n = 5) housed for 30 days in space (Bion-flight = BF) compared to muscles from Bion biosatellite on the ground-housed animals (Bion ground = BG) and from standard cage housed animals (Flight control = FC). A comparison study was carried out with muscles of rats subjected to hindlimb unloading (HU). Gene array and qPCR results showed an increase in Homer1a transcripts, the short dominant negative isoform, in soleus (SOL) muscle after 30 days in microgravity, whereas it was only transiently increased after four days of HU. Conversely, Homer2 long-form was downregulated in SOL muscle in both models. Homer immunofluorescence intensity analysis at the NMJ of BF and HU animals showed comparable outcomes in SOL but not in the extensor digitorum longus (EDL) muscle. Reduced Homer crosslinking at the NMJ consequent to increased Homer1a and/or reduced Homer2 may contribute to muscle-type specific atrophy resulting from microgravity and HU disuse suggesting mutual mechanisms.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Homer isoform switch
en
dc.subject
NMJ adaptation
en
dc.subject
microgravity
en
dc.subject
hindlimb unloading
en
dc.subject
muscle atrophy
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Reciprocal Homer1a and Homer2 Isoform Expression Is a Key Mechanism for Muscle Soleus Atrophy in Spaceflown Mice
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
75
dcterms.bibliographicCitation.doi
10.3390/ijms23010075
dcterms.bibliographicCitation.journaltitle
International Journal of Molecular Sciences
dcterms.bibliographicCitation.number
1
dcterms.bibliographicCitation.originalpublishername
MDPI AG
dcterms.bibliographicCitation.volume
23
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
35008503
dcterms.isPartOf.eissn
1422-0067