dc.contributor.author
Tegtmeyer, Nicole
dc.contributor.author
Esmaeili, Delara Soltan
dc.contributor.author
Sharafutdinov, Irshad
dc.contributor.author
Knorr, Jakob
dc.contributor.author
Naumann, Michael
dc.contributor.author
Alter, Thomas
dc.contributor.author
Backert, Steffen
dc.date.accessioned
2022-02-28T09:19:21Z
dc.date.available
2022-02-28T09:19:21Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/34210
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-33928
dc.description.abstract
Transcription factors of the nuclear factor kappa‐light‐chain‐enhancer of activated B cells (NF-ĸB) family control important signaling pathways in the regulation of the host innate immune system. Various bacterial pathogens in the human gastrointestinal tract induce NF-ĸB activity and provoke pro-inflammatory signaling events in infected epithelial cells. NF-ĸB activation requires the phosphorylation-dependent proteolysis of inhibitor of ĸB (IĸB) molecules including the NF-ĸB precursors through ubiquitin-mediated proteolysis. The canonical NF-ĸB pathway merges on IĸB kinases (IKKs), which are required for signal transduction. Using CRISPR-Cas9 technology, secreted embryonic alkaline phosphatase (SEAP) reporter assays and cytokine enzyme-linked immunosorbent assay (ELISA), we demonstrate that the actin-binding protein cortactin is involved in NF-ĸB activation and subsequent interleukin-8 (IL-8) production upon infection by Helicobacter pylori, Salmonella enterica and Pseudomonas aeruginosa. Our data indicate that cortactin is needed to efficiently activate the c-Sarcoma (Src) kinase, which can positively stimulate NF-ĸB during infection. In contrast, cortactin is not involved in activation of NF-ĸB and IL-8 expression upon infection with Campylobacter species C. jejuni, C. coli or C. consisus, suggesting that Campylobacter species pluralis (spp.) induce a different signaling pathway upstream of cortactin to trigger the innate immune response.
en
dc.format.extent
9 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by-nc/4.0/
dc.subject
Helicobacter pylori
en
dc.subject
interleukin-8
en
dc.subject
inflammation
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie
dc.title
Importance of cortactin for efficient epithelial NF-ĸB activation by Helicobacter pylori, Salmonella enterica and Pseudomonas aeruginosa, but not Campylobacter spp.
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.doi
10.1556/1886.2021.00023
dcterms.bibliographicCitation.journaltitle
European Journal of Microbiology and Immunology
dcterms.bibliographicCitation.number
4
dcterms.bibliographicCitation.pagestart
95
dcterms.bibliographicCitation.pageend
103
dcterms.bibliographicCitation.volume
11
dcterms.bibliographicCitation.url
https://doi.org/10.1556/1886.2021.00023
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Lebensmittelsicherheit und -hygiene

refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
2062-8633
refubium.resourceType.provider
WoS-Alert