dc.contributor.author
Golian, Marek
dc.contributor.author
Bien, Tanja
dc.contributor.author
Schmelzle, Sebastian
dc.contributor.author
Esparza-Mora, Margy Alejandra
dc.contributor.author
McMahon, Dino Peter
dc.contributor.author
Dreisewerd, Klaus
dc.contributor.author
Buellesbach, Jan
dc.date.accessioned
2022-02-03T15:14:17Z
dc.date.available
2022-02-03T15:14:17Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/33880
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-33599
dc.description.abstract
Most of our knowledge on insect cuticular hydrocarbons (CHCs) stems from analytical techniques based on gas-chromatography coupled with mass spectrometry (GC-MS). However, this method has its limits under standard conditions, particularly in detecting compounds beyond a chain length of around C40. Here, we compare the CHC chain length range detectable by GC-MS with the range assessed by silver-assisted laser desorption/ionization mass spectrometry (Ag-LDI-MS), a novel and rarely applied technique on insect CHCs, in seven species of the order Blattodea. For all tested species, we unveiled a considerable range of very long-chain CHCs up to C58, which are not detectable by standard GC-MS technology. This indicates that general studies on insect CHCs may frequently miss compounds in this range, and we encourage future studies to implement analytical techniques extending the conventionally accessed chain length range. Furthermore, we incorporate 3D scanned insect body surface areas as an additional factor for the comparative quantification of extracted CHC amounts between our study species. CHC quantity distributions differed considerably when adjusted for body surface areas as opposed to directly assessing extracted CHC amounts, suggesting that a more accurate evaluation of relative CHC quantities can be achieved by taking body surface areas into account.
en
dc.format.extent
13 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
cuticular hydrocarbons
en
dc.subject
Chemical ecology
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie
dc.subject.ddc
500 Naturwissenschaften und Mathematik::590 Tiere (Zoologie)::590 Tiere (Zoologie)
dc.title
Neglected Very Long-Chain Hydrocarbons and the Incorporation of Body Surface Area Metrics Reveal Novel Perspectives for Cuticular Profile Analysis in Insects
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
83
dcterms.bibliographicCitation.doi
10.3390/insects13010083
dcterms.bibliographicCitation.journaltitle
Insects
dcterms.bibliographicCitation.number
1
dcterms.bibliographicCitation.originalpublishername
MDPI
dcterms.bibliographicCitation.volume
13
dcterms.bibliographicCitation.url
https://doi.org/10.3390/insects13010083
refubium.affiliation
Biologie, Chemie, Pharmazie
refubium.affiliation.other
Institut für Biologie
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
2075-4450