dc.contributor.author
Li, Erqin
dc.contributor.author
Zhang, Hao
dc.contributor.author
Jiang, Henan
dc.contributor.author
Pieterse, Corné M. J.
dc.contributor.author
Jousset, Alexandre
dc.contributor.author
Bakker, Peter A. H. M.
dc.contributor.author
Jonge, Ronnie de
dc.date.accessioned
2021-11-25T12:22:17Z
dc.date.available
2021-11-25T12:22:17Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/32856
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-32582
dc.description.abstract
Beneficial plant root-associated microorganisms carry out a range of functions that are essential for plant performance. Establishment of a bacterium on plant roots, however, requires overcoming several challenges, including competition with neighboring microorganisms and host immunity. Forward and reverse genetics have led to the identification of mechanisms that are used by beneficial microorganisms to overcome these challenges, such as the production of iron-chelating compounds, the formation of strong biofilms, or the concealment of characteristic microbial molecular patterns that trigger the host immune system. However, how such mechanisms arose from an evolutionary perspective is much less understood. To study bacterial adaptation in the rhizosphere, we employed experimental evolution to track the physiological and genetic dynamics of root-dwelling Pseudomonas protegens in the Arabidopsis thaliana rhizosphere under axenic conditions. This simplified binary one plant/one bacterium system allows for the amplification of key adaptive mechanisms for bacterial rhizosphere colonization. We identified 35 mutations, including single-nucleotide polymorphisms, insertions, and deletions, distributed over 28 genes. We found that mutations in genes encoding global regulators and in genes for siderophore production, cell surface decoration, attachment, and motility accumulated in parallel, underlining the finding that bacterial adaptation to the rhizosphere follows multiple strategies. Notably, we observed that motility increased in parallel across multiple independent evolutionary lines. All together, these results underscore the strength of experimental evolution in identifying key genes, pathways, and processes for bacterial rhizosphere colonization and a methodology for the development of elite beneficial microorganisms with enhanced root-colonizing capacities that can support sustainable agriculture in the future.
IMPORTANCE
Beneficial root-associated microorganisms carry out many functions that are essential for plant performance. Establishment of a bacterium on plant roots, however, requires overcoming many challenges. Previously, diverse mechanisms that are used by beneficial microorganisms to overcome these challenges were identified. However, how such mechanisms have developed from an evolutionary perspective is much less understood. Here, we employed experimental evolution to track the evolutionary dynamics of a root-dwelling pseudomonad on the root of Arabidopsis. We found that mutations in global regulators, as well as in genes for siderophore production, cell surface decoration, attachment, and motility, accumulate in parallel, emphasizing these strategies for bacterial adaptation to the rhizosphere. We identified 35 mutations distributed over 28 genes. All together, our results demonstrate the power of experimental evolution in identifying key pathways for rhizosphere colonization and a methodology for the development of elite beneficial microorganisms that can support sustainable agriculture.
en
dc.format.extent
21 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
beneficial microorganisms
en
dc.subject
experimental evolution
en
dc.subject
microbial genomics
en
dc.subject
rhizosphere-inhabiting microbes
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::580 Pflanzen (Botanik)::580 Pflanzen (Botanik)
dc.title
Experimental-Evolution-Driven Identification of Arabidopsis Rhizosphere Competence Genes in Pseudomonas protegens
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
e00927-21
dcterms.bibliographicCitation.doi
10.1128/mBio.00927-21
dcterms.bibliographicCitation.journaltitle
mBio
dcterms.bibliographicCitation.number
3
dcterms.bibliographicCitation.volume
12
dcterms.bibliographicCitation.url
https://doi.org/10.1128/mBio.00927-21
refubium.affiliation
Biologie, Chemie, Pharmazie
refubium.affiliation.other
Institut für Biologie

refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
2150-7511
refubium.resourceType.provider
WoS-Alert