dc.contributor.author
Greuel, Selina
dc.contributor.author
Hanci, Güngör
dc.contributor.author
Böhme, Mike
dc.contributor.author
Miki, Toshio
dc.contributor.author
Schubert, Frank
dc.contributor.author
Sittinger, Michael
dc.contributor.author
Mandenius, Carl‐Fredrik
dc.contributor.author
Zeilinger, Katrin
dc.contributor.author
Freyer, Nora
dc.date.accessioned
2021-11-18T15:00:22Z
dc.date.available
2021-11-18T15:00:22Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/32775
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-32501
dc.description.abstract
Objective: For optimized expansion of human-induced pluripotent stem cells (hiPSCs) with regards to clinical applications, we investigated the influence of the inoculum density on the expansion procedure in 3D hollow-fibre bioreactors.
Materials and Methods: Analytical-scale bioreactors with a cell compartment volume of 3 mL or a large-scale bioreactor with a cell compartment volume of 17 mL were used and inoculated with either 10 x 10(6) or 50 x 10(6) hiPSCs. Cells were cultured in bioreactors over 15 days; daily measurements of biochemical parameters were performed. At the end of the experiment, the CellTiter-Blue (R) Assay was used for culture activity evaluation and cell quantification. Also, cell compartment sections were removed for gene expression and immunohistochemistry analysis.
Results: The results revealed significantly higher values for cell metabolism, cell activity and cell yields when using the higher inoculation number, but also a more distinct differentiation. As large inoculation numbers require cost and time-extensive pre-expansion, low inoculation numbers may be used preferably for long-term expansion of hiPSCs. Expansion of hiPSCs in the large-scale bioreactor led to a successful production of 5.4 x 10(9) hiPSCs, thereby achieving sufficient cell amounts for clinical applications.
Conclusions: In conclusion, the results show a significant effect of the inoculum density on cell expansion, differentiation and production of hiPSCs, emphasizing the importance of the inoculum density for downstream applications of hiPSCs. Furthermore, the bioreactor technology was successfully applied for controlled and scalable production of hiPSCs for clinical use.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
bioreactor culture
en
dc.subject
cell expansion
en
dc.subject
human-induced pluripotent stem cells
en
dc.subject
inoculum density
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Effect of inoculum density on human‐induced pluripotent stem cell expansion in 3D bioreactors
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
e12604
dcterms.bibliographicCitation.doi
10.1111/cpr.12604
dcterms.bibliographicCitation.journaltitle
Cell Proliferation
dcterms.bibliographicCitation.number
4
dcterms.bibliographicCitation.originalpublishername
Wiley
dcterms.bibliographicCitation.volume
52
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.funding
DEAL Wiley
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
31069891
dcterms.isPartOf.issn
0960-7722
dcterms.isPartOf.eissn
1365-2184