dc.contributor.author
Ullah, Imran
dc.contributor.author
Busch, Jonas Felix
dc.contributor.author
Rabien, Anja
dc.contributor.author
Ergün, Bettina
dc.contributor.author
Stamm, Christof
dc.contributor.author
Knosalla, Christoph
dc.contributor.author
Hippenstiel, Stefan
dc.contributor.author
Reinke, Petra
dc.contributor.author
Kurtz, Andreas
dc.date.accessioned
2021-11-05T10:18:13Z
dc.date.available
2021-11-05T10:18:13Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/32577
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-32301
dc.description.abstract
The selection of pluripotent stem cell (PSC)-derived cells for tissue modeling and cell therapy will be influenced by their response to the tissue environment, including the extracellular matrix (ECM). Whether and how instructive memory is imprinted in adult ECM and able to impact on the tissue specific determination of human PSC-derived developmentally fetal mesodermal precursor (P-meso) cells is investigated. Decellularized ECM (dECM) is generated from human heart, kidney, and lung tissues and recellularized with P-meso cells in a medium not containing any differentiation inducing components. While P-meso cells on kidney dECM differentiate exclusively into nephronal cells, only beating clusters containing mature and immature cardiac cells form on heart dECM. No tissue-specific differentiation of P-meso cells is observed on endoderm-derived lung dECM. P-meso-derived endothelial cells, however, are found on all dECM preparations independent of tissue origin. Clearance of heparan-sulfate proteoglycans (HSPG) from dECM abolishes induction of tissue-specific differentiation. It is concluded that HSPG-bound factors on adult tissue-derived ECM are essential and sufficient to induce tissue-specific specification of uncommitted fetal stage precursor cells.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
cell differentiation
en
dc.subject
extracellular matrix
en
dc.subject
pluripotent stem cells
en
dc.subject
regenerative medicine
en
dc.subject
tissue engineering
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Adult Tissue Extracellular Matrix Determines Tissue Specification of Human iPSC‐Derived Embryonic Stage Mesodermal Precursor Cells
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
1901198
dcterms.bibliographicCitation.doi
10.1002/advs.201901198
dcterms.bibliographicCitation.journaltitle
Advanced Science
dcterms.bibliographicCitation.number
5
dcterms.bibliographicCitation.originalpublishername
Wiley
dcterms.bibliographicCitation.volume
7
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.funding
DEAL Wiley
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
32154066
dcterms.isPartOf.eissn
2198-3844