dc.contributor.author
Härtel, Heike
dc.contributor.author
Theiß, Janine
dc.contributor.author
Abdelaziz, Mohammed O.
dc.contributor.author
Raftery, Martin J.
dc.contributor.author
Pecher, Gabriele
dc.contributor.author
Bogner, Elke
dc.date.accessioned
2021-09-28T11:34:32Z
dc.date.available
2021-09-28T11:34:32Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/32089
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-31817
dc.description.abstract
Human cytomegalovirus (HCMV) has been implicated in the development of human malignancies, for instance in colon cancer. Proteasome inhibitors were developed for cancer therapy and have also been shown to influence HCMV infection. The aim of this study was to investigate if proteasome inhibitors have therapeutic potential for colon carcinoma and how this is influenced by HCMV infection. We show by immunofluorescence and flow cytometry that the colon carcinoma cell line Caco-2 is susceptible to HCMV infection. Growth curve analysis as well as protein expression kinetics and quantitative genome analysis further confirm these results. HCMV has an anti-apoptotic effect on Caco-2 cells by inhibiting very early events of the apoptosis cascade. Further investigations showed that HCMV stabilizes the membrane potential of the mitochondria, which is typically lost very early during apoptosis. This stabilization is resistant to proteasome inhibitor Bortezomib treatment, allowing HCMV-infected cells to survive apoptotic signals. Our findings indicate a possible role of proteasome inhibitors in colon carcinoma therapy.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
human cytomegalovirus
en
dc.subject
Caco-2 cells
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
HCMV-Mediated Interference of Bortezomib-Induced Apoptosis in Colon Carcinoma Cell Line Caco-2
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
83
dcterms.bibliographicCitation.doi
10.3390/v13010083
dcterms.bibliographicCitation.journaltitle
Viruses
dcterms.bibliographicCitation.number
1
dcterms.bibliographicCitation.originalpublishername
MDPI AG
dcterms.bibliographicCitation.volume
13
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
33435377
dcterms.isPartOf.eissn
1999-4915