dc.contributor.author
Nattramilarasu, Praveen Kumar
dc.contributor.author
Lobo de Sá, Fábia Daniela
dc.contributor.author
Schulzke, Jörg-Dieter
dc.contributor.author
Bücker, Roland
dc.date.accessioned
2021-09-23T10:15:09Z
dc.date.available
2021-09-23T10:15:09Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/32033
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-31762
dc.description.abstract
Campylobacter concisus is a human-pathogenic bacterium of the gastrointestinal tract. This study aimed at the contribution of the mucosal immune system in the context of intestinal epithelial barrier dysfunction induced by C. concisus. As an experimental leaky gut model, we used in vitro co-cultures of colonic epithelial cell monolayers (HT-29/B6-GR/MR) with M1-macrophage-like THP-1 cells on the basal side. Forty-eight hours after C. concisus infection, the decrease in the transepithelial electrical resistance in cell monolayers was more pronounced in co-culture condition and 22 ± 2% (p < 0.001) higher than the monoculture condition without THP-1 cells. Concomitantly, we observed a reduction in the expression of the tight junction proteins occludin and tricellulin. We also detected a profound increase in 4 kDa FITC-dextran permeability in C. concisus-infected cell monolayers only in co-culture conditions. This is explained by loss of tricellulin from tricellular tight junctions (tTJs) after C. concisus infection. As an underlying mechanism, we observed an inflammatory response after C. concisus infection through pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) released from THP-1 cells in the co-culture condition. In conclusion, the activation of subepithelial immune cells exacerbates colonic epithelial barrier dysfunction by C. concisus through tricellulin disruption in tTJs, leading to increased antigen permeability (leaky gut concept).
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Campylobacter concisus
en
dc.subject
HT-29/B6-GR/MR cells
en
dc.subject
leaky gut model
en
dc.subject
tricellular tight junction
en
dc.subject
tumor necrosis factor-α (TNF-α)
en
dc.subject
interleukin-1β (IL-1β)
en
dc.subject
interleukin-6 (IL-6)
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Immune-Mediated Aggravation of the Campylobacter concisus-Induced Epithelial Barrier Dysfunction
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
2043
dcterms.bibliographicCitation.doi
10.3390/ijms22042043
dcterms.bibliographicCitation.journaltitle
International Journal of Molecular Sciences
dcterms.bibliographicCitation.number
4
dcterms.bibliographicCitation.originalpublishername
MDPI AG
dcterms.bibliographicCitation.volume
22
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
33669494
dcterms.isPartOf.eissn
1422-0067