dc.contributor.author
Urbantat, Ruth
dc.contributor.author
Blank, Anne
dc.contributor.author
Kremenetskaia, Irina
dc.contributor.author
Vajkoczy, Peter
dc.contributor.author
Acker, Güliz
dc.contributor.author
Brandenburg, Susan
dc.date.accessioned
2021-09-15T13:17:13Z
dc.date.available
2021-09-15T13:17:13Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/31970
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-31701
dc.description.abstract
We aimed to evaluate the angiogenic capacity of CXCL2 and IL8 affecting human endothelial cells to clarify their potential role in glioblastoma (GBM) angiogenesis. Human GBM samples and controls were stained for proangiogenic factors. Survival curves and molecule correlations were obtained from the TCGA (The Cancer Genome Atlas) database. Moreover, proliferative, migratory and angiogenic activity of peripheral (HUVEC) and brain specific (HBMEC) primary human endothelial cells were investigated including blockage of CXCR2 signaling with SB225502. Gene expression analyses of angiogenic molecules from endothelial cells were performed. Overexpression of VEGF and CXCL2 was observed in GBM patients and associated with a survival disadvantage. Molecules of the VEGF pathway correlated but no relation for CXCR1/2 and CXCL2/IL8 was found. Interestingly, receptors of endothelial cells were not induced by addition of proangiogenic factors in vitro. Proliferation and migration of HUVEC were increased by VEGF, CXCL2 as well as IL8. Their sprouting was enhanced through VEGF and CXCL2, while IL8 showed no effect. In contrast, brain endothelial cells reacted to all proangiogenic molecules. Additionally, treatment with a CXCR2 antagonist led to reduced chemokinesis and sprouting of endothelial cells. We demonstrate the impact of CXCR2 signaling on endothelial cells supporting an impact of this pathway in angiogenesis of glioblastoma.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
tumor angiogenesis
en
dc.subject
glioblastoma
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
The CXCL2/IL8/CXCR2 Pathway Is Relevant for Brain Tumor Malignancy and Endothelial Cell Function
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
2634
dcterms.bibliographicCitation.doi
10.3390/ijms22052634
dcterms.bibliographicCitation.journaltitle
International Journal of Molecular Sciences
dcterms.bibliographicCitation.number
5
dcterms.bibliographicCitation.originalpublishername
MDPI AG
dcterms.bibliographicCitation.volume
22
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
33807899
dcterms.isPartOf.eissn
1422-0067