dc.contributor.author
You, Yu
dc.contributor.author
Hagag, Ibrahim T.
dc.contributor.author
Kheimar, Ahmed
dc.contributor.author
Bertzbach, Luca D.
dc.contributor.author
Kaufer, Benedikt B.
dc.date.accessioned
2021-11-11T14:17:42Z
dc.date.available
2021-11-11T14:17:42Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/31445
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-31178
dc.description.abstract
Marek’s disease virus (MDV) is a highly cell-associated oncogenic alphaherpesvirus that causes lymphomas in various organs in chickens. Like other herpesviruses, MDV has a large and complex double-stranded DNA genome. A number of viral transcripts are generated by alternative splicing, a process that drastically extends the coding capacity of the MDV genome. One of the spliced genes encoded by MDV is the viral interleukin 8 (vIL-8), a CXC chemokine that facilitates the recruitment of MDV target cells and thereby plays an important role in MDV pathogenesis and tumorigenesis. We recently identified a novel vIL-8 exon (vIL-8-E3′) by RNA-seq; however, it remained elusive whether the protein containing the vIL-8-E3′ is expressed and what role it may play in MDV replication and/or pathogenesis. To address these questions, we first generated recombinant MDV harboring a tag that allows identification of the spliced vIL-8-E3′ protein, revealing that it is indeed expressed. We subsequently generated knockout viruses and could demonstrate that the vIL-8-E3′ protein is dispensable for MDV replication as well as secretion of the functional vIL-8 chemokine. Finally, infection of chickens with this vIL-8-E3′ knockout virus revealed that the protein is not important for MDV replication and pathogenesis in vivo. Taken together, our study provides novel insights into the splice forms of the CXC chemokine of this highly oncogenic alphaherpesvirus.
en
dc.format.extent
12 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Marek’s disease virus
en
dc.subject
viral chemokine
en
dc.subject
alternative RNA splicing
en
dc.subject
splice acceptor site
en
dc.subject
pathogenesis
en
dc.subject
tumorigenesis
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::636 Viehwirtschaft
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::616 Krankheiten
dc.title
Characterization of a Novel Viral Interleukin 8 (vIL-8) Splice Variant Encoded by Marek’s Disease Virus
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
1475
dcterms.bibliographicCitation.doi
10.3390/microorganisms9071475
dcterms.bibliographicCitation.journaltitle
Microorganisms
dcterms.bibliographicCitation.number
7
dcterms.bibliographicCitation.originalpublishername
MDPI
dcterms.bibliographicCitation.volume
9
dcterms.bibliographicCitation.url
https://doi.org/10.3390/microorganisms9071475
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Virologie

refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
2076-2607