dc.contributor.author
Bhattacharyya, Aritra
dc.contributor.author
Herta, Toni
dc.contributor.author
Conrad, Claudia
dc.contributor.author
Frey, Doris
dc.contributor.author
García, Pedro
dc.contributor.author
Suttorp, Norbert
dc.contributor.author
Hippenstiel, Stefan
dc.contributor.author
Zahlten, Janine
dc.date.accessioned
2021-05-27T13:44:22Z
dc.date.available
2021-05-27T13:44:22Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/30889
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-30628
dc.description.abstract
The recruitment and activation of polymorphonuclear neutrophils (PMNs) are of central importance for the elimination of pathogens in bacterial infections. We investigated the Streptococcus pneumoniae-dependent induction of the transcription factor Kruppel-like factor (KLF) 4 in PMNs as a potential regulator of PMN activation. We found that KLF4 expression is induced in human blood-derived PMNs in a time- and dose-dependent manner by wild-type S. pneumoniae and capsule knockout mutants. Unencapsulated knockout mutants induced stronger KLF4 expression than encapsulated wild types. The presence of autolysin LytA-competent (thus viable) pneumococci and LytA-mediated bacterial autolysis were required for KLF4 induction in human and murine PMNs. LyzMcre-mediated knockdown of KLF4 in murine blood-derived PMNs revealed that KLF4 influences pneumococci killing and increases the release of the proinflammatory cytokines tumor necrosis factor alpha and keratinocyte chemoattractant and decreases the release of the anti-inflammatory cytokine interleukin-10. Thus, S. pneumoniae induces KLF4 expression in PMNs, which contributes to PMN activation in S. pneumoniae infection.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
infectious diseases
en
dc.subject
innate immunity
en
dc.subject
Krüppel-like factor 4
en
dc.subject
S. pneumoniae
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Induction of Krüppel-Like Factor 4 Mediates Polymorphonuclear Neutrophil Activation in Streptococcus pneumoniae Infection
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
582070
dcterms.bibliographicCitation.doi
10.3389/fmicb.2020.582070
dcterms.bibliographicCitation.journaltitle
Frontiers in Microbiology
dcterms.bibliographicCitation.originalpublishername
Frontiers Media SA
dcterms.bibliographicCitation.volume
11
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
33613460
dcterms.isPartOf.eissn
1664-302X