dc.contributor.author
Hering, Nina A.
dc.contributor.author
Luettig, Julia
dc.contributor.author
Jebautzke, Britta
dc.contributor.author
Schulzke, Jörg D.
dc.contributor.author
Rosenthal, Rita
dc.date.accessioned
2021-05-18T06:33:56Z
dc.date.available
2021-05-18T06:33:56Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/30801
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-30540
dc.description.abstract
Scope: Ellagitannins are polyphenols found in numerous fruits, nuts and seeds. The elagitannin punicalagin and its bioactive metabolites ellagic acid and urolithins are discussed to comprise a high potential for therapeutically or preventive medical application such as in intestinal diseases. The present study characterizes effects of punicalagin, ellagic acid and urolithin A on intestinal barrier function in the absence or presence of the proinflammatory cytokine tumor necrosis factor-alpha (TNF alpha).
Methods and Results: Transepithelial resistance (TER), fluorescein and ion permeability, tight junction protein expression and signalling pathways were examined in Caco-2 and HT-29/B6 intestinal epithelial cell models. Punicalagin had less or no effects on barrier function in both cell models. Ellagic acid was most effective in ileum-like Caco-2 cells, where it increased TER and reduced fluorescein and sodium permeabilities. This was paralleled by myosin light chain kinase two mediated expression down-regulation of claudin-4, -7 and -15. Urolithin A impeded the TNF alpha-induced barrier loss by inhibition of claudin-1 and -2 protein expression upregulation and claudin-1 delocalization in HT-29/B6.
Conclusion: Ellagic acid and urolithin A affect intestinal barrier function in distinct ways. Ellagic acid acts preventive by strengthening the barrier per se, while urolithin A protects against inflammation-induced barrier dysfunction.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
barrier function
en
dc.subject
ellagic acid
en
dc.subject
tight junction
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
The Punicalagin Metabolites Ellagic Acid and Urolithin A Exert Different Strengthening and Anti-Inflammatory Effects on Tight Junction-Mediated Intestinal Barrier Function In Vitro
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
610164
dcterms.bibliographicCitation.doi
10.3389/fphar.2021.610164
dcterms.bibliographicCitation.journaltitle
Frontiers in Pharmacology
dcterms.bibliographicCitation.originalpublishername
Frontiers Media SA
dcterms.bibliographicCitation.volume
12
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
33776763
dcterms.isPartOf.eissn
1663-9812