dc.contributor.author
Reisbeck, Felix
dc.contributor.author
Ozimkovski, Alexander
dc.contributor.author
Cherri, Mariam
dc.contributor.author
Dimde, Mathias
dc.contributor.author
Quaas, Elisa
dc.contributor.author
Mohammadifar, Ehsan
dc.contributor.author
Achazi, Katharina
dc.contributor.author
Haag, Rainer
dc.date.accessioned
2021-04-22T10:09:43Z
dc.date.available
2021-04-22T10:09:43Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/30485
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-30225
dc.description.abstract
Biocompatible polymers with the ability to load and release a cargo at the site of action in a smart response to stimuli have attracted great attention in the field of drug delivery and cancer therapy. In this work, we synthesize a dual-responsive dendritic polyglycerol sulfate (DR-dPGS) drug delivery system by copolymerization of glycidol, ε-caprolactone and an epoxide monomer bearing a disulfide bond (SSG), followed by sulfation of terminal hydroxyl groups of the copolymer. The effect of different catalysts, including Lewis acids and organic bases, on the molecular weight, monomer content and polymer structure was investigated. The degradation of the polymer backbone was proven in presence of reducing agents and candida antarctica Lipase B (CALB) enzyme, which results in the cleavage of the disulfides and ester bonds, respectively. The hydrophobic anticancer drug Doxorubicin (DOX) was loaded in the polymer and the kinetic assessment showed an enhanced drug release with glutathione (GSH) or CALB as compared to controls and a synergistic effect of a combination of both stimuli. Cell uptake was studied by using confocal laser scanning microscopy with HeLa cells and showed the uptake of the Dox-loaded carriers and the release of the drug into the nucleus. Cytotoxicity tests with three different cancer cell lines showed good tolerability of the polymers of as high concentrations as 1 mg mL−1, while cancer cell growth was efficiently inhibited by DR-dPGS@Dox.
en
dc.format.extent
14 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
drug delivery system
en
dc.subject
dual-responsiveness
en
dc.subject
dendritic polyglycerol sulfates
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::540 Chemie::540 Chemie und zugeordnete Wissenschaften
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::616 Krankheiten
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::615 Pharmakologie, Therapeutik
dc.title
Gram Scale Synthesis of Dual-Responsive Dendritic Polyglycerol Sulfate as Drug Delivery System
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
982
dcterms.bibliographicCitation.doi
10.3390/polym13060982
dcterms.bibliographicCitation.journaltitle
Polymers
dcterms.bibliographicCitation.number
6
dcterms.bibliographicCitation.originalpublishername
MDPI
dcterms.bibliographicCitation.volume
13
dcterms.bibliographicCitation.url
https://doi.org/10.3390/polym13060982
refubium.affiliation
Biologie, Chemie, Pharmazie
refubium.affiliation.other
Institut für Chemie und Biochemie
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refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
2073-4360