dc.contributor.author
Keshavan, Sandeep
dc.contributor.author
Andón, Fernando Torres
dc.contributor.author
Gallud, Audrey
dc.contributor.author
Chen, Wei
dc.contributor.author
Reinert, Knut
dc.contributor.author
Tran, Lang
dc.contributor.author
Fadeel, Bengt
dc.date.accessioned
2021-04-19T13:47:40Z
dc.date.available
2021-04-19T13:47:40Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/30427
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-30168
dc.description.abstract
Engineered nanomaterials are potentially very useful for a variety of applications, but studies are needed to ascertain whether these materials pose a risk to human health. Here, we studied three benchmark nanomaterials (Ag nanoparticles, TiO2 nanoparticles, and multi-walled carbon nanotubes, MWCNTs) procured from the nanomaterial repository at the Joint Research Centre of the European Commission. Having established a sub-lethal concentration of these materials using two human cell lines representative of the immune system and the lungs, respectively, we performed RNA sequencing of the macrophage-like cell line after exposure for 6, 12, and 24 h. Downstream analysis of the transcriptomics data revealed significant effects on chemokine signaling pathways. CCR2 was identified as the most significantly upregulated gene in MWCNT-exposed cells. Using multiplex assays to evaluate cytokine and chemokine secretion, we could show significant effects of MWCNTs on several chemokines, including CCL2, a ligand of CCR2. The results demonstrate the importance of evaluating sub-lethal concentrations of nanomaterials in relevant target cells.
en
dc.format.extent
16 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
multi-walled carbon nanotubes
en
dc.subject
nanoparticles
en
dc.subject
transcriptomics
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Profiling of Sub-Lethal in Vitro Effects of Multi-Walled Carbon Nanotubes Reveals Changes in Chemokines and Chemokine Receptors
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
883
dcterms.bibliographicCitation.doi
10.3390/nano11040883
dcterms.bibliographicCitation.journaltitle
Nanomaterials
dcterms.bibliographicCitation.number
4
dcterms.bibliographicCitation.originalpublishername
MDPI
dcterms.bibliographicCitation.volume
11
dcterms.bibliographicCitation.url
https://doi.org/10.3390/nano11040883
refubium.affiliation
Mathematik und Informatik
refubium.affiliation.other
Institut für Informatik
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
2079-4991