dc.contributor.author
van der Mierden, Stevie
dc.contributor.author
Leenaars, Cathalijn H. C.
dc.contributor.author
Boyle, Erin C.
dc.contributor.author
Ripoli, Florenza L.
dc.contributor.author
Gass, Peter
dc.contributor.author
Durst, Mattea
dc.contributor.author
Goerlich-Jansson, Vivian C.
dc.contributor.author
Jirkof, Paulin
dc.contributor.author
Keubler, Lydia M.
dc.contributor.author
Lewejohann, Lars
dc.date.accessioned
2021-04-16T10:39:58Z
dc.date.available
2021-04-16T10:39:58Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/30392
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-30133
dc.description.abstract
Evaluating stress in laboratory animals is a key principle in animal welfare. Measuring corticosterone is a common method to assess stress in laboratory mice. There are, however, numerous methods to measure glucocorticoids with differences in sample matrix (e.g., plasma, urine) and quantification techniques (e.g., enzyme immunoassay or radioimmunoassay). Here, the authors present a mapping review and a searchable database, giving a complete overview of all studies measuring endogenous corticosterone in mice up to February 2018. For each study, information was recorded regarding mouse strain and sex; corticosterone sample matrix and quantification technique; and whether the study covered the research theme animal welfare, neuroscience, stress, inflammation, or pain (the themes of specific interest in our consortium). Using all database entries for the year 2012, an exploratory meta-regression was performed to determine the effect of predictors on basal corticosterone concentrations. Seventy-five studies were included using the predictors sex, time-since-lights-on, sample matrix, quantification technique, age of the mice, and type of control. Sex, time-since-lights-on, and type of control significantly affected basal corticosterone concentrations. The resulting database can be used, inter alia, for preventing unnecessary duplication of experiments, identifying knowledge gaps, and standardizing or heterogenizing methodologies. These results will help plan more efficient and valid experiments in the future and can answer new questions in silico using meta-analyses.
en
dc.format.extent
12 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
mapping review
en
dc.subject
corticosterone
en
dc.subject
meta-regression
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie
dc.title
Measuring Endogenous Corticosterone in Laboratory Mice - a Mapping Review, Meta-Analysis, and Open Source Database
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.doi
10.14573/altex.2004221
dcterms.bibliographicCitation.journaltitle
ALTEX - Alternatives to Animal Experimentation
dcterms.bibliographicCitation.number
1
dcterms.bibliographicCitation.pagestart
111
dcterms.bibliographicCitation.pageend
122
dcterms.bibliographicCitation.volume
38
dcterms.bibliographicCitation.url
https://doi.org/10.14573/altex.2004221
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Tierschutz, Tierverhalten und Versuchstierkunde
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refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.issn
1868-596X
dcterms.isPartOf.eissn
1868-8551
refubium.resourceType.provider
WoS-Alert