dc.contributor.author
Lemmnitzer, Katharina
dc.contributor.author
Köhling, Sebastian
dc.contributor.author
Freyse, Joanna
dc.contributor.author
Rademann, Jörg
dc.contributor.author
Schiller, Jürgen
dc.date.accessioned
2021-04-15T10:03:22Z
dc.date.available
2021-04-15T10:03:22Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/30356
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-30097
dc.description.abstract
Glycosaminoglycans (GAG) as long, unbranched polysaccharides are major components of the extracellular matrix. Many studies provided additional evidence of a specific binding between mediators and sulfated GAG, at which the sulfation code-which means the number and positions of sulfate groups along the polysaccharide chain-plays an important role.
GAG from natural sources are very inhomogeneous regarding their sulfation patterns and molecular weight. Additionally, there is a high risk of contamination. This results in a growing interest in the careful characterization of native GAG and the synthesis of artificial GAG. Additionally, chemically oversulfated GAG analogues show many favorable properties. However, the structural characterization of these carbohydrates by mass spectrometry remains challenging. One significant problem is the sulfate loss during the ionization, which increases with the number of sulfate residues.
We used the sulfated pentasaccharide fondaparinux as model substance to optimize sample preparation and measurement conditions, compared different established desalination methods and already existing protocols for sulfated oligosaccharides, and investigated their impact on the quality of the mass spectra. After optimization of the measurement conditions, we could establish a gentle and fast protocol for the mass spectrometry characterization of (fully) sulfated, artificial GAG-like oligosaccharides with minimized sulfate loss in the positive and negative ion mode. Here, the negative ion mode was more sensitive in comparison with the positive one, and fondaparinux species with sulfate loss were not detectable under the optimized conditions in the positive ion mode.
en
dc.format.extent
11 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject
(over)sulfation
en
dc.subject
desalination
en
dc.subject
fondaparinux
en
dc.subject
glycosaminoglycans
en
dc.subject
sulfate loss
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie
dc.title
Characterization of defined sulfated heparin-like oligosaccharides by electrospray ionization ion trap mass spectrometry
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
e4692
dcterms.bibliographicCitation.doi
10.1002/jms.4692
dcterms.bibliographicCitation.journaltitle
Journal of Mass Spectrometry
dcterms.bibliographicCitation.number
2
dcterms.bibliographicCitation.volume
56
dcterms.bibliographicCitation.url
https://doi.org/10.1002/jms.4692
refubium.affiliation
Biologie, Chemie, Pharmazie
refubium.affiliation.other
Institut für Pharmazie
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1096-9888
refubium.resourceType.provider
WoS-Alert