dc.contributor.author
Sun, Zhiyong
dc.contributor.author
Zhao, Qingcai
dc.contributor.author
Haag, Rainer
dc.contributor.author
Wu, Changzhu
dc.date.accessioned
2021-04-07T13:17:53Z
dc.date.available
2021-04-07T13:17:53Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/30233
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-29974
dc.description.abstract
Multienzyme cascade biocatalysis is an efficient synthetic process, avoiding the isolation/purification of intermediates and shifting the reaction equilibrium to the product side. However, multienzyme systems are often limited by their incompatibility and cross‐reactivity. Herein, we report a multi‐responsive emulsion to proceed multienzyme reactions sequentially for high reactivity. The emulsion is achieved using a CO2, pH, and thermo‐responsive block copolymer as a stabilizer, allowing the on‐demand control of emulsion morphology and phase composition. Applying this system to a three‐step cascade reaction enables the individual optimal condition for each enzyme, and a high overall conversion (ca. 97 % of the calculated limit) is thereby obtained. Moreover, the multi‐responsiveness of the emulsion allows the facile and separate yielding/recycling of products, polymers and active enzymes. Besides, the system could be scaled up with a good yield.
en
dc.format.extent
5 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by-nc/4.0/
dc.subject
biocatalysis
en
dc.subject
block copolymers
en
dc.subject
multienzyme reaction
en
dc.subject
responsive emulsion
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::540 Chemie::540 Chemie und zugeordnete Wissenschaften
dc.title
Responsive Emulsions for Sequential Multienzyme Cascades
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.doi
10.1002/anie.202013737
dcterms.bibliographicCitation.journaltitle
Angewandte Chemie International Edition
dcterms.bibliographicCitation.number
15
dcterms.bibliographicCitation.pagestart
8410
dcterms.bibliographicCitation.pageend
8414
dcterms.bibliographicCitation.volume
60
dcterms.bibliographicCitation.url
https://doi.org/10.1002/anie.202013737
refubium.affiliation
Biologie, Chemie, Pharmazie
refubium.affiliation.other
Institut für Chemie und Biochemie
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refubium.funding
DEAL Wiley
refubium.note.author
Die Publikation wurde aus Open Access Publikationsgeldern der Freien Universität Berlin gefördert.
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1521-3773