Tear film hyperosmolarity induces dry eye syndrome (DES) through transient receptor potential vanilloid type 1 (TRPV1) activation. L-carnitine is a viable therapeutic agent since it protects against this hypertonicity-induced response. Here, we investigated whether L-carnitine inhibits TRPV1 activation by blocking heat- or capsaicin-induced increases in Ca2+ influx or hyperosmotic stress-induced cell volume shrinkage in a human corneal epithelial cell line (HCE-T). Single-cell fluorescence imaging of calcein/AM-loaded cells or fura-2/AM-labeled cells was used to evaluate cell volume changes and intracellular calcium levels, respectively. Planar patch-clamp technique was used to measure whole-cell currents. TRPV1 activation via either capsaicin (20 & mu;mol/L), hyperosmolarity (& AP;450 mosmol/L) or an increase in ambient bath temperature to 43 & DEG;C induced intracellular calcium transients and augmented whole-cell currents, whereas hypertonicity induced cell volume shrinkage. In contrast, either capsazepine (10 & mu;mol/L) or L-carnitine (1-3 mmol/L) reduced all these responses. Taken together, L-carnitine and capsazepine suppress hypertonicity-induced TRPV1 activation by blocking cell volume shrinkage.
View lessPeritoneal carcinomatosis-associated malignant bowel obstruction is a common feature that merits more attention in advanced and recurrent ovarian cancer. Decompressive gastrostomy is one of the most preferred methods to palliate distressing symptoms and maintain patients' quality of life. We retrospectively identified 31 patients with ovarian cancer-associated MBO, who underwent decompressive CT fluoroscopy-guided percutaneous gastrostomy (CT-PG) between September 2015 and April 2023 at our institution. A systematic literature review was conducted for CT-guided gastrostomy in ovarian cancer. Prior to CT-PG, 27 (87%) patients underwent unsuccessful attempts at endoscopic gastrostomy or surgery due to bowel obstruction; a total of 55% had received & GE;3 lines of chemotherapy. CT-PG could be successfully inserted in 25 of 31 (81%) patients without grade 4-5 complications. CT-PG insertion was feasible in 76% of patients with previous unsuccessful attempts of endoscopic gastrostomy. A total of 80% of patients with a successful insertion had considerable symptom relief and could tolerate fluid intake. Mean survival after the procedure was 44.4 days. Chemotherapy could be administered in 7 of 25 (28%) patients following the CT-PG insertion. CT-guided percutaneous gastrostomy is a safe procedure that effectively manages intractable symptoms of bowel obstruction in ovarian cancer. This minimally invasive technique should be emphasised as a routine instrument within the palliative management of MBO.
View lessSimple Summary Cancer often causes long-term physical and psychological impairments. Lifestyle modification and nature-based interventions (NBIs) can have a positive impact on patients' quality of life (QOL). However, there is sparse scientific research on the effects of natural environments in the treatment of cancer patients. Therefore, we integrated intensified nature experiences into the scientifically substantiated therapy approach of an oncology day care clinic program. This study aimed to compare the effects of a nature-based oncology day care clinic intervention to conventional oncology day care clinic intervention. A positive impact of the day care clinic program on QOL, fatigue, and psychological parameters in cancer patients was shown. In addition, NBIs seem to have more pronounced effects, which need to be further proven.Abstract Cancer often causes long-term physical and psychological impairments. Lifestyle modification and nature-based interventions (NBIs) can have a positive impact on patients' quality of life (QOL). This participants-blinded, non-randomized controlled study assessed parameters at weeks 0, 12, and 24, including, as a primary endpoint, QOL in cancer patients on the Functional Assessment of Cancer Therapy-General (FACT-G) at week 12. QOL in breast cancer patients, fatigue, well-being, stress, anxiety/depression, socio-psychological well-being, benefits of nature interaction, insomnia, self-efficacy, mindfulness, and self-compassion were assessed as secondary endpoints. N = 107 cancer patients (96.3% women; 52.5 & PLUSMN; 9.3 years, 80.4% breast cancer) were assigned to either a 12-week nature-based (NDC; n = 56) or conventional (DC; n = 51) oncology day care clinic program, whereby the assignment group was not known to the participants. There was no significant group difference for the primary endpoint. At week 24, QOL, fatigue, mindfulness and self-compassion scores were significantly higher, and at weeks 12 and 24, the insomnia score was significantly lower in NDC compared to DC. In conclusion, this study indicates positive and clinically relevant effects of the program on QOL, fatigue, and psychological parameters. NBIs seem to have a more pronounced effect.
View lessThe usefulness of perfluorocarbon nanoemulsions for the imaging of experimental myocarditis has been demonstrated in a high-field 9.4 Tesla MRI scanner. Our proof-of-concept study investigated the imaging capacity of PFC-based F-19/H-1 MRI in an animal myocarditis model using a clinical field strength of 1.5 Tesla. To induce experimental myocarditis, five male rats (weight similar to 300 g, age similar to 50 days) were treated with one application per week of doxorubicin (2 mg/kg BW) over a period of six weeks. Three control animals received the identical volume of sodium chloride 0.9% instead. Following week six, all animals received a single 4 ml injection of an 20% oil-in-water perfluorooctylbromide nanoemulsion 24 hours prior to in vivo(1)H/F-19 imaging on a 1.5 Tesla MRI. After euthanasia, cardiac histology and immunohistochemistry using CD68/ED1 macrophage antibodies were performed, measuring the inflamed myocardium in & mu;m(2) for further statistical analysis to compare the extent of the inflammation with the F-19-MRI signal intensity. All animals treated with doxorubicin showed a specific signal in the myocardium, while no myocardial signal could be detected in the control group. Additionally, the doxorubicin group showed a significantly higher SNR for F-19 and a stronger CD68/ED1 immunhistoreactivity compared to the control group. This proof-of-concept study demonstrates that perfluorocarbon nanoemulsions could be detected in an in vivo experimental myocarditis model at a currently clinically relevant field strength.
View lessThe Babylonian horoscopes, edited by Rochberg in 1998, contain sets of astronomical and astrological data on dates of, or close to, individuals’ births. Each element of these short and concise texts reflects the astral divinatory practices and astronomical observation developed over the first millennium BC, as well as the latest innovations, including the zodiac as coordinate system and the occurrence of new astrological schemes partly based on it, which had previously remained obscure. In this paper, I present a new interpretation of the astrological terminology and the schemes that underly some of the data in the Babylonian horoscopes.
View lessStandardised terminology in science is important for clarity of interpretation and communication. In invasion science – a dynamic and rapidly evolving discipline – the proliferation of technical terminology has lacked a standardised framework for its development. The result is a convoluted and inconsistent usage of terminology, with various discrepancies in descriptions of damage and interventions. A standardised framework is therefore needed for a clear, universally applicable, and consistent terminology to promote more effective communication across researchers, stakeholders, and policymakers. Inconsistencies in terminology stem from the exponential increase in scientific publications on the patterns and processes of biological invasions authored by experts from various disciplines and countries since the 1990s, as well as publications by legislators and policymakers focusing on practical applications, regulations, and management of resources. Aligning and standardising terminology across stakeholders remains a challenge in invasion science. Here, we review and evaluate the multiple terms used in invasion science (e.g. ‘non-native’, ‘alien’, ‘invasive’ or ‘invader’, ‘exotic’, ‘non-indigenous’, ‘naturalised’, ‘pest’) to propose a more simplified and standardised terminology. The streamlined framework we propose and translate into 28 other languages is based on the terms (i) ‘non-native’, denoting species transported beyond their natural biogeographic range, (ii) ‘established non-native’, i.e. those non-native species that have established self-sustaining populations in their new location(s) in the wild, and (iii) ‘invasive non-native’ – populations of established non-native species that have recently spread or are spreading rapidly in their invaded range actively or passively with or without human mediation. We also highlight the importance of conceptualising ‘spread’ for classifying invasiveness and ‘impact’ for management. Finally, we propose a protocol for classifying populations based on (i) dispersal mechanism, (ii) species origin, (iii) population status, and (iv) impact. Collectively and without introducing new terminology, the framework that we present aims to facilitate effective communication and collaboration in invasion science and management of non-native species.
View lessAnthropogenic activity has irreparably altered the ecological fabric of Earth. The emergence of ecological novelty from diverse drivers of change is an increasingly challenging dimension of ecosystem restoration. At the same time, the restorationist's tool kit continues to grow, including a variety of powerful and increasingly prevalent technologies. Thus, ecosystem restoration finds itself at the center of intersecting challenges. How should we respond to increasingly common emergence of environmental system states with little or no historical precedent, whilst considering the appropriate deployment of potentially consequential and largely untested interventions that may give rise to organisms, system states, and/or processes that are likewise without historical precedent? We use the term bionovelty to encapsulate these intersecting themes and examine the implications of bionovelty for ecological restoration.
View lessWhole Institution Approaches (WIAs) to sustainability in education emphasize the strong link between the socio-physical environment and the learning processes and outcomes. However, there has been a lack of instruments for quantifying the implementation of WIAs based on the experience of learners and educators. In this article, we present the systematic development, pretesting, validation and first application of a novel WIA-Scale (learners: 13 items, educators: 15 items). As part of a large-scale assessment in Germany (school education, vocational education and higher education; n = 2,985), we apply the WIA-Scale and assess how WIAs are related to the effectiveness of sustainability learning. Based on extensive validation, the scale quality was evaluated as high. The application showed that young people who experience more sustainability in line with the WIA feel strongly more motivated and empowered by their educational institution to contribute to sustainability. Also, regression modeling revealed that WIA implementation is the strongest of various predictors of how sustainable learners act beyond the educational institution. Further, educators who experience more sustainability in line with the WIA feel considerably more motivated and report more sustainable behavior. They also view Education for Sustainable Development (ESD) as more relevant and participate more frequently in training on ESD. For future use, we provide the WIA-Scale in its original version, a 7/8-item variant and a one-item proxy. The scale may be used as a process indicator for high-quality sustainability education and as part of organizational development. Overall, the study shows that experienced WIAs are measurable and highly effective for quality sustainability learning.
View lessPurpose
To propose a paradigm change for the validation procedures of medication adherence questionnaires.
Methods
A total of 121 validation procedures of unique questionnaires for medication adherence were analyzed.
Results
“Construct validity” and “internal consistency” were most often assessed, and test results varied largely. A more in-depth analysis indicated that the assessment of medication non-adherence included distinct but related constructs, such as the extent to which doses are missed, and the attempt to identify different facets of medication-taking behavior. Consequently, each construct requires a different measurement approach with different psychometric tests for establishing its validity and reliability.
Conclusion
Results show that assessing the validity and reliability of adherence questionnaires with standard procedures including statistical tests is inconclusive. Refinement of the constructs of non-adherence is needed in pharmacy and medical practice. We suggest a distinction between the (i) extent of missed doses over the past 2 weeks, (ii) modifiable reasons for non-adherence behavior, and (iii) unmodifiable factors of non-adherence. Validation procedures and corresponding statistical methods should be selected according to the specific single constructs.
View lessWe established confrontations between two different fungi, i.e., the maize anthracnose and stalk rot pathogen Colletotrichum graminicola, and the ubiquitous fungus Aspergillus nidulans, and different biocontrol species, i.e., Bacillus subtilis, Bacillus velezensis, and Bacillus amyloliquefaciens. In all fungus–bacterium confrontations tested, growth arrest and, thus, distance inhibition was observed on solid substrata. LC–MS/MS analyses of culture filtrates suggested formation of several metabolites only synthesized in confrontations. Interestingly, microscopy of fungal hyphae grown in liquid medium showed protrusions and color changes occurred only in media harboring fungus-bacterium confrontations. These observations indicate metabolic re-programming and suggest formation of putative secondary metabolites in interactions involving microbial biocontrol agents.
View lessLaser-induced forward transfer (LIFT) of polymers is a versatile printing method for parallel in situ synthesis of peptides on microarrays. Chemical building blocks embedded in a polymer matrix are transferred and coupled in a desired pattern to a surface, generating peptides on microarrays by repetitive in situ solid-phase synthesis steps. To date, the approach is limited to simple, heat induced chemical reactions. The VaporLIFT method, disclosed here, combines LIFT with chemical vapor glycosylation to rapidly generate glycans on microarray surfaces while maintaining inert, low temperature conditions required for glycosylations. Process design and parameter optimization enables the synthesis of a collection of glycans at defined positions on a glass surface. The synthetic structures are detected by mass spectrometry, fluorescently labeled glycan-binding proteins, and covalent staining with fluorescent dyes. VaporLIFT is ideal for parallel screening of other chemical reactions, that require inert and well-defined reaction conditions.
View lessThe monazite-cheralite solid solutions LnPO4-Ca0.5Th0.5PO4 with Ln = La, Gd were prepared via a co-precipitation route, showcasing an optimised, scalable synthesis procedure for a possible waste form accommodating high level liquid waste streams. A distortion of the cheralite structure with respect to the monazite structure was observed throughout both solid solutions as evidenced by a deviation of the lattice parameters from the linear behaviour known from other monazite solid solutions. Using a high temperature flux method, cheralite single crystals were grown for the first time for in-depth structural investigations. Both thorium and calcium were found to deviate from the central position of the LnO9 polyhedron, supporting previous neutron diffraction investigations of identical cheralite samples.
View lessZirconia-based ceramics are promising host matrices for the immobilization of radionuclides in high-level waste streams due to their high radiation resistance and chemical stability. This study explores coprecipitation and different solid-state synthesis techniques to produce phase-pure zirconia-based ceramics with varying cerium and neodymium co-doping. Varying the dopant concentration enabled the synthesis of zirconates with monoclinic, cubic defect fluorite, and cubic pyrochlore structures. Powder X-ray diffraction was used for phase identification. In the case of coprecipitation, all synthesized compositions were predominantly phase-pure. Solid-state synthesis techniques included manual mixing of metal oxide powders with mortar and pestle, mechanical mixing in a ball mill, and magnetic mixing in a slurry. All solid-state mixing methods produced heterogeneous ceramics, featuring multiple phases, with manual mixing yielding the most phase-pure product. Extending the grinding time, re-sintering of the solid phases, and an increased Nd content were found to enhance the phase purity.
View lessThis article undertakes a crucial examination of Kant’s depiction of the interconnectedness between human reason and the divine. The argument posits that Kant conceptualizes reason as inherently founded on a divine basis. The primary objective of this article is not to delve into whether Kant endorses or dismisses specific Christian doctrines, or if his portrayal of reason aligns with a particular strand of Christian thought. Instead, the aim is to chart an interpretive middle path – one that embraces the profound philosophical underpinnings embedded in Kant’s rationalist legacy while recognizing the inherent limitations he imposes on human reason. The conclusion drawn is that Kant’s account of reason tends towards the divine, though the precise nature of this interconnectedness remains shrouded in mystery.
View lessThe self-identification of Sweden and Canada as exceptionally caring and ethical states committed to the goal of global gender justice has been reinforced through their adoption of feminist foreign policies (FFPs). However, despite the enthusiasm surrounding the potential of feminism as an ethical approach to foreign policy making, critics have drawn attention to the many failings of FFPs that hamper their ability to bring about transformative change. This article posits that the shortcomings of Swedish and Canadian FFPs are rooted in an unacknowledged legacy of colonial power relations. It is from within this history of violence that Swedish and Canadian practices of feminism both “at home” and “abroad” can be seen as a re-articulation of colonialism. Through two illustrative narrative readings of reconciliation efforts, the article examines state provisions of hierarchically imposed, paternalistic expressions of care that silence Indigenous “articulations of self-care.” As such, it addresses a significant gap in the literature on FFPs by highlighting the risk of self-proclaimed “caring” feminist states reproducing colonial power relations within and beyond borders.
View lessThis study investigates the determinants and dynamics of the linguistic features of key audit matters (KAMs) in European countries. Using natural language processing algorithms, including FINBERT, I quantify stylistic and content-based text characteristics at the KAM level and find that KAM length, readability, sentiment, quantitative density, specificity, the degree of forward-looking statements, and the extent of boilerplate language are associated with the type of KAM topics, client attributes, and audit firm characteristics. In additional analyses, I also find early empirical evidence of a time trend in these linguistic features. Since their introduction in 2016, KAMs are becoming longer, more quantitative, more specific, but also include more boilerplate phrases. Collectively, the results of the study contribute to a more nuanced understanding of the determinants and dynamics of KAM disclosures.
View lessTo contribute to a better understanding of the determinants of climate-friendly organizational behavior, we study the potential of behavioral policy interventions and social norms to foster individual contributions to organizational decarbonization initiatives. We investigate the effects of different types of behavioral policy interventions (default nudges vs. short-term boosts) in isolation and when they are combined with normative appeals to adopt climate-friendly behaviors in an organizational context. In a 2 × 2 between-subjects experiment, we find that default nudges generally induced higher individual contributions to organizational carbon compensation programs than short-term boosts. Moreover, injunctive social norm information decreased the effectiveness of both types of behavioral interventions but affected the effectiveness of short-term boosts to a stronger extent than the effectiveness of default nudges. Contributing to the nascent literature on motivating climate change mitigating behaviors in organizational contexts, we additionally explore whether factors such as personality traits, pro-social and pro-environmental beliefs, attitudes, and behaviors, and the degree of organizational identification exert an influence on the effectiveness of the interventions and provide qualitative insights into participants’ reasoning for their decisions.
View less