dc.contributor.author
Klees, Sylvia
dc.contributor.author
Effelsberg, Natalie
dc.contributor.author
Stührenberg, Birgit
dc.contributor.author
Mellmann, Alexander
dc.contributor.author
Schwarz, Stefan
dc.contributor.author
Köck, Robin
dc.date.accessioned
2021-02-18T13:17:20Z
dc.date.available
2021-02-18T13:17:20Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/29682
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-29426
dc.description.abstract
The surveillance of antimicrobial resistance among humans and food-producing animals is important to monitor the zoonotic transmission of multidrug-resistant bacteria (MDRB). We assessed the prevalence of four MDRB within the meat production chain, including extended-spectrum β-lactamase (ESBL)-producing, carbapenemase-producing Enterobacterales (CPE) and colistin-resistant Enterobacterales (Col-E), as well as vancomycin-resistant enterococci (VRE). In total, 505 samples from four stages of meat production, i.e., slaughterhouses, meat-processing plants, fresh food products and the urban environment, were collected in northwestern Germany in 2018/2019 and screened for the presence of MDRB using both culture-based and PCR-based techniques. We detected genes encoding for carbapenemases in 9–56% (blaOXA-48, blaKPC, blaNDM, blaVIM) and colistin resistance-encoding mcr genes in 9–26% of the samples from all stages. Culture-based analysis found CPE and VRE only in environmental samples (11% and 7%, respectively), but Col-E and ESBL-producers in 1–7% and 12–46% of samples from all stages, respectively. Overall, our results showed that ESBL-producers and mcr-carrying Col-E were common in food-producing animals at slaughterhouses, in meat-processing plants and in food items at retail, while CPE and VRE were only found in the environment. The discrepancy between detected carbapenemase genes and isolated CPE emphasizes the need for more sensitive detection methods for CPE monitoring.
en
dc.format.extent
14 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
antimicrobial resistance
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::616 Krankheiten
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::636 Viehwirtschaft
dc.title
Prevalence and Epidemiology of Multidrug-Resistant Pathogens in the Food Chain and the Urban Environment in Northwestern Germany
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
708
dcterms.bibliographicCitation.doi
10.3390/antibiotics9100708
dcterms.bibliographicCitation.journaltitle
Antibiotics
dcterms.bibliographicCitation.number
10
dcterms.bibliographicCitation.originalpublishername
MDPI
dcterms.bibliographicCitation.volume
9
dcterms.bibliographicCitation.url
https://doi.org/10.3390/antibiotics9100708
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Mikrobiologie und Tierseuchen
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
2079-6382