dc.contributor.author
Wight, Darren J.
dc.contributor.author
Aimola, Giulia
dc.contributor.author
Aswad, Amr
dc.contributor.author
Lai, Chi-Yu Jill
dc.contributor.author
Bahamon, Christian
dc.contributor.author
Hong, Karl
dc.contributor.author
Hill, Joshua A.
dc.contributor.author
Kaufer, Benedikt B.
dc.date.accessioned
2021-02-08T14:38:32Z
dc.date.available
2021-02-08T14:38:32Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/29543
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-29287
dc.description.abstract
Next-generation sequencing technologies allowed sequencing of thousands of genomes. However, there are genomic regions that remain difficult to characterize, including telomeres, centromeres, and other low-complexity regions, as well as transposable elements and endogenous viruses. Human herpesvirus 6A and 6B (HHV-6A and HHV-6B) are closely related viruses that infect most humans and can integrate their genomes into the telomeres of infected cells. Integration also occurs in germ cells, meaning that the virus can be inherited and result in individuals harboring the virus in every cell of their body. The integrated virus can reactivate and cause disease in humans. While it is well established that the virus resides in the telomere region, the integration locus is poorly defined due to the low sequence complexity (TTAGGG)n of telomeres that cannot be easily resolved through sequencing. We therefore employed genome imaging of the integrated HHV-6A and HHV-6B genomes using whole-genome optical site mapping technology. Using this technology, we identified which chromosome arm harbors the virus genome and obtained a high resolution map of the integration loci of multiple patients. Surprisingly, this revealed long telomere sequences at the virus-subtelomere junction that were previously missed using PCR-based approaches. Contrary to what was previously thought, our technique revealed that the telomere lengths of chromosomes harbor ing the integrated virus genome were comparable to the other chromosomes. Taken together, our data shed light on the genetic structure of the HHV-6A and HHV-6B integration locus, demonstrating the utility of optical mapping for the analysis of genomic regions that are difficult to sequence.
en
dc.format.extent
7 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject
human herpesvirus 6
en
dc.subject
telomere integration
en
dc.subject
structural genomic mapping
en
dc.subject
virus integration
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie
dc.title
Unbiased optical mapping of telomere-integrated endogenous human herpesvirus 6
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.doi
10.1073/pnas.2011872117
dcterms.bibliographicCitation.journaltitle
Proceedings of the National Academy of Sciences (PNAS)
dcterms.bibliographicCitation.number
49
dcterms.bibliographicCitation.pagestart
31410
dcterms.bibliographicCitation.pageend
31416
dcterms.bibliographicCitation.volume
117
dcterms.bibliographicCitation.url
https://doi.org/10.1073/pnas.2011872117
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Virologie
![This authority value has been confirmed as accurate by an interactive user](/cache_202e45ad85b55efaeb29160f63cd3f3b/themes/FuCD/images/authority_control/invisible.gif)
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.issn
0027-8424
dcterms.isPartOf.eissn
1091-6490
refubium.resourceType.provider
WoS-Alert