dc.contributor.author
Johanns, Vanessa C.
dc.contributor.author
Epping, Lennard
dc.contributor.author
Semmler, Torsten
dc.contributor.author
Ghazisaeedi, Fereshteh
dc.contributor.author
Lübke-Becker, Antina
dc.contributor.author
Pfeifer, Yvonne
dc.contributor.author
Eichhorn, Inga
dc.contributor.author
Merle, Roswitha
dc.contributor.author
Bethe, Astrid
dc.contributor.author
Walther, Birgit
dc.date.accessioned
2021-02-05T15:13:16Z
dc.date.available
2021-02-05T15:13:16Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/29518
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-29262
dc.description.abstract
To prevent economic losses due to post-weaning diarrhea (PWD) in industrial pig production, zinc (Zn) feed additives have been widely used, especially since awareness has risen that the regular application of antibiotics promotes buildup of antimicrobial resistance in both commensal and pathogenic bacteria. In a previous study on 179 Escherichia coli collected from piglets sacrificed at the end of a Zn feeding trial, including isolates obtained from animals of a high-zinc fed group (HZG) and a corresponding control group (CG), we found that the isolate collection exhibited three different levels of tolerance toward zinc, i.e., the minimal inhibitory concentration (MIC) detected was 128, followed by 256 and 512 mu g/ml ZnCl2. We further provided evidence that enhanced zinc tolerance in porcine intestinal E. coli populations is clearly linked to excessive zinc feeding. Here we provide insights about the genomic make-up and phylogenetic background of these 179 E. coli genomes. Bayesian analysis of the population structure (BAPS) revealed a lack of association between the actual zinc tolerance level and a particular phylogenetic E. coli cluster or even branch for both, isolates belonging to the HZG and CG. In addition, detection rates for genes and operons associated with virulence (VAG) and bacteriocins (BAG) were lower in isolates originating from the HZG (41 vs. 65% and 22 vs. 35%, p < 0.001 and p = 0.002, resp.). Strikingly, E. coli harboring genes defining distinct pathotypes associated with intestinal disease, i.e., enterotoxigenic, enteropathogenic, and Shiga toxin-producing E. coli (ETEC, EPEC, and STEC) constituted 1% of the isolates belonging to the HZG but 14% of those from the CG. Notably, these pathotypes were positively associated with enhanced zinc tolerance (512 mu g/ml ZnCl2 MIC, p < 0.001). Taken together, zinc excess seems to influence carriage rates of VAGs and BAGs in porcine intestinal E. coli populations, and high-zinc feeding is negatively correlated with enteral pathotype occurrences, which might explain earlier observations concerning the relative increase of Enterobacterales considering the overall intestinal microbiota of piglets during zinc feeding trials while PWD rates have decreased.
en
dc.format.extent
13 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
virulence associated genes
en
dc.subject
bacteriocins
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
dc.title
High-Zinc Supplementation of Weaned Piglets Affects Frequencies of Virulence and Bacteriocin Associated Genes Among Intestinal Escherichia coli Populations
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
614513
dcterms.bibliographicCitation.doi
10.3389/fvets.2020.614513
dcterms.bibliographicCitation.journaltitle
Frontiers in Veterinary Science
dcterms.bibliographicCitation.volume
7
dcterms.bibliographicCitation.url
https://doi.org/10.3389/fvets.2020.614513
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Mikrobiologie und Tierseuchen

refubium.affiliation.other
Institut für Veterinär-Epidemiologie und Biometrie

refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
2297-1769
refubium.resourceType.provider
WoS-Alert