dc.contributor.author
Markov, Alexander G.
dc.contributor.author
Fedorova, Arina A.
dc.contributor.author
Kravtsova, Violetta V.
dc.contributor.author
Bikmurzina, Anastasia E.
dc.contributor.author
Okorokova, Larisa S.
dc.contributor.author
Matchkov, Vladimir V.
dc.contributor.author
Cornelius, Valeria
dc.contributor.author
Amasheh, Salah
dc.contributor.author
Krivoi, Igor I.
dc.date.accessioned
2021-02-05T13:51:04Z
dc.date.available
2021-02-05T13:51:04Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/29508
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-29252
dc.description.abstract
The ability of exogenous low ouabain concentrations to affect claudin expression and therefore epithelial barrier properties was demonstrated previously in cultured cell studies. We hypothesized that chronic elevation of circulating ouabain in vivo can affect the expression of claudins and tight junction permeability in different tissues. We tested this hypothesis in rats intraperitoneally injected with ouabain (1 μg/kg) for 4 days. Rat jejunum, colon and brain frontal lobes, which are variable in the expressed claudins and tight junction permeability, were examined. Moreover, the porcine jejunum cell line IPEC-J2 was studied. In IPEC-J2-cells, ouabain (10 nM, 19 days of incubation) stimulated epithelial barrier formation, increased transepithelial resistance and the level of cSrc-kinase activation by phosphorylation, accompanied with an increased expression of claudin-1, -5 and down-regulation of claudin-12; the expression of claudin-3, -4, -8 and tricellulin was not changed. In the jejunum, chronic ouabain increased the expression of claudin-1, -3 and -5 without an effect on claudin-2 and -4 expression. In the colon, only down-regulation of claudin-3 was observed. Chronic ouabain protected the intestine transepithelial resistance against functional injury induced by lipopolysaccharide treatment or by modeled acute microgravity; this regulation was most pronounced in the jejunum. Claudin-1 was also up-regulated in cerebral blood vessels. This was associated with reduction of claudin-3 expression while the expression of claudin-5 and occludin was not affected. Altogether, our results confirm that circulating ouabain can functionally and tissue-specifically affect barrier properties of epithelial and endothelial tissues via Na,K-ATPase-mediated modulation of claudins expression.
en
dc.format.extent
16 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
circulating ouabain
en
dc.subject
brain blood vessels
en
dc.subject
IPEC-J2 cells
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::616 Krankheiten
dc.title
Circulating Ouabain Modulates Expression of Claudins in Rat Intestine and Cerebral Blood Vessels
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
5067
dcterms.bibliographicCitation.doi
10.3390/ijms21145067
dcterms.bibliographicCitation.journaltitle
International Journal of Molecular Sciences
dcterms.bibliographicCitation.number
14
dcterms.bibliographicCitation.originalpublishername
MDPI
dcterms.bibliographicCitation.volume
21
dcterms.bibliographicCitation.url
https://doi.org/10.3390/ijms21145067
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Veterinär-Physiologie

refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1422-0067