dc.contributor.author
Tkhilaishvili, Tamta
dc.contributor.author
Wang, Lei
dc.contributor.author
Perka, Carsten
dc.contributor.author
Trampuz, Andrej
dc.contributor.author
Gonzalez Moreno, Mercedes
dc.date.accessioned
2021-01-28T15:24:40Z
dc.date.available
2021-01-28T15:24:40Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/29383
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-29129
dc.description.abstract
Pseudomonas aeruginosa and Staphylococcus aureus are pathogens able to colonize surfaces and form together a mixed biofilm. Dual-species biofilms are significantly more resistant to antimicrobials than a monomicrobial community, leading to treatment failure. Due to their rapid bactericidal activity, the self-amplification ability and the biofilm degrading properties, bacteriophages represent a promising therapeutic option in fighting biofilm-related infections. In this study, we investigated the effect of either the simultaneous or staggered application of commercially available phages and ciprofloxacin versus S. aureus/P. aeruginosa dual-species biofilms in vitro. Biofilms were grown on porous glass beads and analyzed over time. Different techniques such as microcalorimetry, sonication and scanning electron microscopy were combined for the evaluation of anti-biofilm activities. Both bacterial species were susceptible to ciprofloxacin and to phages in their planktonic form of growth. Ciprofloxacin tested alone against biofilms required high concentration ranging from 256 to >512 mg/L to show an inhibitory effect, whereas phages alone showed good and moderate activity against MRSA biofilms and dual-species biofilms, respectively, but low activity against P. aeruginosa biofilms. The combination of ciprofloxacin with phages showed a remarkable improvement in the anti-biofilm activity of both antimicrobials with complete eradication of dual-species biofilms after staggered exposure to Pyophage or Pyophage + Staphylococcal phage for 12 h followed by 1 mg/L of ciprofloxacin, a dose achievable by intravenous or oral antibiotic administration. Our study provides also valuable data regarding not only dosage but also an optimal time of antimicrobial exposure, which is crucial in the implementation of combined therapies.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Pseudomonas aeruginosa
en
dc.subject
methicillin-resistant Staphylococcus aureus
en
dc.subject
biofilm-associated infection
en
dc.subject
dual-species biofilm
en
dc.subject
antibiotic-bacteriophage combination
en
dc.subject
bacteriophages
en
dc.subject
isothermal microcalorimetry
en
dc.subject
scanning electron microscopy
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Using Bacteriophages as a Trojan Horse to the Killing of Dual-Species Biofilm Formed by Pseudomonas aeruginosa and Methicillin Resistant Staphylococcus aureus
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
695
dcterms.bibliographicCitation.doi
10.3389/fmicb.2020.00695
dcterms.bibliographicCitation.journaltitle
Frontiers in Microbiology
dcterms.bibliographicCitation.originalpublishername
Frontiers Media SA
dcterms.bibliographicCitation.volume
11
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
32351494
dcterms.isPartOf.eissn
1664-302X