dc.contributor.author
Kløve, Sigri
dc.contributor.author
Genger, Claudia
dc.contributor.author
Weschka, Dennis
dc.contributor.author
Mousavi, Soraya
dc.contributor.author
Bereswill, Stefan
dc.contributor.author
Heimesaat, Markus M.
dc.date.accessioned
2021-01-18T14:14:44Z
dc.date.available
2021-01-18T14:14:44Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/29012
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-28762
dc.description.abstract
Human Campylobacter infections are emerging worldwide and constitute significant health burdens. We recently showed that the immunopathological sequelae in Campylobacter jejuni-infected mice were due to Toll-like receptor (TLR)-4 dependent immune responses induced by bacterial lipooligosaccharide (LOS). Information regarding the molecular mechanisms underlying Campylobacter coli-host interactions are scarce, however. Therefore, we analyzed C. coli-induced campylobacteriosis in secondary abiotic IL-10-/- mice with and without TLR4. Mice were infected perorally with a human C. coli isolate or with a murine commensal Escherichia coli as apathogenic, non-invasive control. Independent from TLR4, C. coli and E. coli stably colonized the gastrointestinal tract, but only C. coli induced clinical signs of campylobacteriosis. TLR4-/- IL-10-/- mice, however, displayed less frequently fecal blood and less distinct histopathological and apoptotic sequelae in the colon versus IL-10-/- counterparts on day 28 following C. coli infection. Furthermore, C. coli-induced colonic immune cell responses were less pronounced in TLR4-/- IL-10-/- as compared to IL-10-/- mice and accompanied by lower pro-inflammatory mediator concentrations in the intestines and the liver of the former versus the latter. In conclusion, our study provides evidence that TLR4 is involved in mediating C. coli-LOS-induced immune responses in intestinal and extra-intestinal compartments during murine campylobacteriosis.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Toll-like receptor-4
en
dc.subject
lipooligosaccharide
en
dc.subject
Campylobacter coli
en
dc.subject
campylobacteriosis model
en
dc.subject
host-pathogen interaction
en
dc.subject
secondary abiotic IL-10−/− mice
en
dc.subject
pro-inflammatory immune responses
en
dc.subject
intestinal immunopathology
en
dc.subject
extra-intestinal immune responses
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Toll-Like Receptor-4 Is Involved in Mediating Intestinal and Extra-Intestinal Inflammation in Campylobacter coli-Infected Secondary Abiotic IL-10−/− Mice
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
1882
dcterms.bibliographicCitation.doi
10.3390/microorganisms8121882
dcterms.bibliographicCitation.journaltitle
Microorganisms
dcterms.bibliographicCitation.number
12
dcterms.bibliographicCitation.originalpublishername
MDPI AG
dcterms.bibliographicCitation.volume
8
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
33261211
dcterms.isPartOf.eissn
2076-2607