dc.contributor.author
Keller, Sarah
dc.contributor.author
Borde, Tabea
dc.contributor.author
Brangsch, Julia
dc.contributor.author
Adams, Lisa C.
dc.contributor.author
Kader, Avan
dc.contributor.author
Reimann, Carolin
dc.contributor.author
Gebert, Pimrapat
dc.contributor.author
Hamm, Bernd
dc.contributor.author
Makowski, Marcus
dc.date.accessioned
2020-11-12T13:40:53Z
dc.date.available
2020-11-12T13:40:53Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/28772
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-28521
dc.description.abstract
To characterize the tumor extracellular matrix (ECM) using native T1 mapping magnetic resonance imaging (MRI) in an experimental hepatic cancer model, a total of 27 female New Zealand white rabbits with hepatic VX2 tumors were examined by MRI at different time points following tumor implantation (day 14, 21, 28). A steady-state precession readout single-shot MOLLI sequence was acquired in a 3 T MRI scanner in prone position using a head-neck coil. The tumors were segmented into a central, marginal, and peritumoral region in anatomical images and color-coded T1 maps. In histopathological sections, stained with H&E and Picrosirius red, the regions corresponded to central tumor necrosis and accumulation of viable cells with fibrosis in the tumor periphery. Another region of interest (ROI) was placed in healthy liver tissue. T1 times were correlated with quantitative data of collagen area staining. A two-way repeated-measures ANOVA was used to compare cohorts and tumor regions. Hepatic tumors were successfully induced in all rabbits. T1 mapping demonstrated significant differences between the different tumor regions (F(1.43,34.26) = 106.93, p < 0.001) without interaction effects between time points and regions (F(2.86,34.26) = 0.74, p = 0.53). In vivo T1 times significantly correlated with ex vivo collagen stains (area %), (center: r = 0.78, p < 0.001; margin: r = 0.84, p < 0.001; peritumoral: r = 0.73, p < 0.001). Post hoc tests using Sidak's correction revealed significant differences in T1 times between all three regions (p < 0.001). Native T1 mapping is feasible and allows the differentiation of tumor regions based on ECM composition in a longitudinal tumor study in an experimental small animal model, making it a potential quantitative biomarker of ECM remodeling and a promising technique for future treatment studies.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
magnetic resonance imaging
en
dc.subject
extracellular matrix
en
dc.subject
liver cancer
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Native T1 Mapping Magnetic Resonance Imaging as a Quantitative Biomarker for the Characterization of the Extracellular Matrix in a Rabbit Hepatic Cancer Model
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
412
dcterms.bibliographicCitation.doi
10.3390/biomedicines8100412
dcterms.bibliographicCitation.journaltitle
Biomedicines
dcterms.bibliographicCitation.number
10
dcterms.bibliographicCitation.originalpublishername
MDPI AG
dcterms.bibliographicCitation.volume
8
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
33066169
dcterms.isPartOf.eissn
2227-9059