dc.contributor.author
Claussen, Felix
dc.contributor.author
Gassner, Joseph M. G. V.
dc.contributor.author
Moosburner, Simon
dc.contributor.author
Wyrwal, David
dc.contributor.author
Nösser, Maximilian
dc.contributor.author
Tang, Peter
dc.contributor.author
Wegener, Lara
dc.contributor.author
Pohl, Julian
dc.contributor.author
Reutzel-Selke, Anja
dc.contributor.author
Arsenic, Ruza
dc.contributor.author
Pratschke, Johann
dc.contributor.author
Sauer, Igor M.
dc.contributor.author
Raschzok, Nathanael
dc.date.accessioned
2021-01-20T09:03:28Z
dc.date.available
2021-01-20T09:03:28Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/28726
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-28474
dc.description.abstract
Background: Normothermic ex vivo liver perfusion (NEVLP) is a promising strategy to increase the donor pool in liver transplantation. Small animal models are essential to further investigate questions regarding organ preservation and reconditioning by NEVLP. A dual vessel small animal NEVLP (dNEVLP) model was developed using metamizole as a vasodilator and compared to conventional portovenous single vessel NEVLP (sNEVLP).
Methods: Livers of male Wistar rats were perfused with erythrocyte-supplemented culture medium for six hours by either dNEVLP via hepatic artery and portal vein or portovenous sNEVLP. dNEVLP was performed either with or without metamizole treatment. Perfusion pressure and flow rates were constantly monitored. Transaminase levels were determined in the perfusate at the start and after three and six hours of perfusion. Bile secretion was monitored and bile LDH and GGT levels were measured hourly. Histopathological analysis was performed using liver and bile duct tissue samples after perfusion.
Results: Hepatic artery pressure was significantly lower in dNEVLP with metamizole administration. Compared to sNEVLP, dNEVLP with metamizole treatment showed higher bile production, lower levels of transaminases during and after perfusion as well as significantly lower necrosis in liver and bile duct tissue. Biochemical markers of bile duct injury showed the same trend.
Conclusion: Our miniaturized dNEVLP system enables normothermic dual vessel rat liver perfusion. The administration of metamizole effectively ameliorates arterial vasospasm allowing for six hours of dNEVLP, with superior outcome compared to sNEVLP.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Arterial Pressure
en
dc.subject
Hepatic Artery
en
dc.subject
Organ Preservation
en
dc.subject
Vasodilation
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Dual versus single vessel normothermic ex vivo perfusion of rat liver grafts using metamizole for vasodilatation
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
e0235635
dcterms.bibliographicCitation.doi
10.1371/journal.pone.0235635
dcterms.bibliographicCitation.journaltitle
PLOS ONE
dcterms.bibliographicCitation.number
7
dcterms.bibliographicCitation.originalpublishername
Public Library of Science (PLoS)
dcterms.bibliographicCitation.volume
15
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
32614897
dcterms.isPartOf.eissn
1932-6203