dc.contributor.author
Mieloch, Adam Aron
dc.contributor.author
Zurawek, Magdalena
dc.contributor.author
Giersig, Michael
dc.contributor.author
Rozwadowska, Natalia
dc.contributor.author
Rybka, Jakub Dalibor
dc.date.accessioned
2020-09-29T07:31:51Z
dc.date.available
2020-09-29T07:31:51Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/28401
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-28151
dc.description.abstract
Superparamagnetic iron oxide nanoparticles (SPIONs) have been investigated for wide variety of applications. Their unique properties render them highly applicable as MRI contrast agents, in magnetic hyperthermia or targeted drug delivery. SPIONs surface properties affect a whole array of parameters such as: solubility, toxicity, stability, biodistribution etc. Therefore, progress in the field of SPIONs surface functionalization is crucial for further development of therapeutic or diagnostic agents. In this study, SPIONs were synthesized by thermal decomposition of iron (III) acetylacetonate Fe(acac) 3 and functionalized with dihexadecyl phosphate (DHP) via phase transfer. Bioactivity of the SPION-DHP was assessed on SW1353 and TCam-2 cancer derived cell lines. The following test were conducted: cytotoxicity and proliferation assay, reactive oxygen species (ROS) assay, SPIONs uptake (via Iron Staining and ICP-MS), expression analysis of the following genes: alkaline phosphatase (ALPL); ferritin light chain (FTL); serine/threonine protein phosphatase 2A (PP2A); protein tyrosine phosphatase non-receptor type 11 (PTPN11); transferrin receptor 1 (TFRC) via RT-qPCR. SPION-DHP nanoparticles were successfully obtained and did not reveal significant cytotoxicity in the range of tested concentrations. ROS generation was elevated, however not correlated with the concentrations. Gene expression profile was slightly altered only in SW1353 cells.
en
dc.format.extent
11 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
tyrosine phosphatases
en
dc.subject
transferrin receptor
en
dc.subject
protein corona
en
dc.subject
biodegradation
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::530 Physik::530 Physik
dc.title
Bioevaluation of superparamagnetic iron oxide nanoparticles (SPIONs) functionalized with dihexadecyl phosphate (DHP)
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
2725
dcterms.bibliographicCitation.doi
10.1038/s41598-020-59478-2
dcterms.bibliographicCitation.journaltitle
Scientific Reports
dcterms.bibliographicCitation.number
1
dcterms.bibliographicCitation.volume
10
dcterms.bibliographicCitation.url
https://doi.org/10.1038/s41598-020-59478-2
refubium.affiliation
Physik
refubium.affiliation.other
Institut für Experimentalphysik

refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
2045-2322
refubium.resourceType.provider
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