dc.contributor.author
Pustlauk, Wera
dc.contributor.author
Westhoff, Timm H.
dc.contributor.author
Claeys, Luc
dc.contributor.author
Roch, Toralf
dc.contributor.author
Geißler, Sven
dc.contributor.author
Babel, Nina
dc.date.accessioned
2020-08-20T10:40:41Z
dc.date.available
2020-08-20T10:40:41Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/28092
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-27842
dc.description.abstract
Vascular calcification is a severe pathological event in the manifestation of atherosclerosis. Pathogenic triggers mediating osteogenic differentiation of arterial smooth muscle cells (SMC) in humans remain insufficiently understood and are to a large extent investigated in animal models or cells derived thereof. Here, we describe an in vitro model based on SMC derived from healthy and diseased humans that allows to comprehensively investigate vascular calcification mechanisms. Comparing the impact of the commonly used SMC culture media VascuLife, DMEM, and M199, cells were characterised by immunofluorescence, flow cytometry, qPCR, and regarding their contractility and proliferative capacity. Irrespective of the arterial origin, the clinical background and the expansion medium used, all cells expressed typical molecular SMC marker while contractility varied between donors. Interestingly, the ability to induce an osteogenic differentiation strongly depended on the culture medium, with only SMC cultured in DMEM depositing calcified matrix upon osteogenic stimulation, which correlated with increased alkaline phosphatase activity, increased inorganic phosphate level and upregulation of osteogenic gene markers. Our optimized model is suitable for donor-oriented as well as broader screening of potential pathogenic mediators triggering vascular calcification. Translational studies aiming to identify and to evaluate therapeutic targets in a personalized fashion would be feasible.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
smooth muscle cells
en
dc.subject
vascular calcification
en
dc.subject
atherosclerosis
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Induced osteogenic differentiation of human smooth muscle cells as a model of vascular calcification
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
5951
dcterms.bibliographicCitation.doi
10.1038/s41598-020-62568-w
dcterms.bibliographicCitation.journaltitle
Scientific Reports
dcterms.bibliographicCitation.originalpublishername
Nature Research
dcterms.bibliographicCitation.volume
10
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
32249802
dcterms.isPartOf.eissn
2045-2322