dc.contributor.author
Stich, Stefan
dc.contributor.author
Jagielski, Michal
dc.contributor.author
Fleischmann, Anja
dc.contributor.author
Meier, Carola
dc.contributor.author
Bussmann, Patricia
dc.contributor.author
Kohl, Benjamin
dc.contributor.author
Schmidt, Julia
dc.contributor.author
Krüger, Jan-Philipp
dc.contributor.author
Endres, Michaela
dc.contributor.author
Cabraja, Mario
dc.contributor.author
Reimann, Kolja
dc.contributor.author
Laue, Dominik
dc.contributor.author
Ertel, Wolfgang
dc.contributor.author
Sittinger, Michael
dc.date.accessioned
2020-08-24T12:22:52Z
dc.date.available
2020-08-24T12:22:52Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/28075
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-27825
dc.description.abstract
Intervertebral disc (IVD) herniation and degeneration is a major source of back pain. In order to regenerate a herniated and degenerated disc, closure of the anulus fibrosus (AF) is of crucial importance. For molecular characterization of AF, genome-wide Affymetrix HG-U133plus2.0 microarrays of native AF and cultured cells were investigated. To evaluate if cells derived from degenerated AF are able to initiate gene expression of a regenerative pattern of extracellular matrix (ECM) molecules, cultivated cells were stimulated with bone morphogenetic protein 2 (BMP2), transforming growth factor β1 (TGFβ1) or tumor necrosis factor-α (TNFα) for 24 h. Comparative microarray analysis of native AF tissues showed 788 genes with a significantly different gene expression with 213 genes more highly expressed in mild and 575 genes in severe degenerated AF tissue. Mild degenerated native AF tissues showed a higher gene expression of common cartilage ECM genes, whereas severe degenerated AF tissues expressed genes known from degenerative processes, including matrix metalloproteinases (MMP) and bone associated genes. During monolayer cultivation, only 164 differentially expressed genes were found. The cells dedifferentiated and altered their gene expression profile. RTD-PCR analyses of BMP2- and TGFβ1-stimulated cells from mild and severe degenerated AF tissue after 24 h showed an increased expression of cartilage associated genes. TNFα stimulation increased MMP1, 3, and 13 expression. Cells derived from mild and severe degenerated tissues could be stimulated to a comparable extent. These results give hope that regeneration of mildly but also strongly degenerated disc tissue is possible.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
intervertebral disc
en
dc.subject
anulus fibrosus
en
dc.subject
degeneration
en
dc.subject
genome-wide microarray
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Degeneration of Lumbar Intervertebral Discs: Characterization of Anulus Fibrosus Tissue and Cells of Different Degeneration Grades
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
2165
dcterms.bibliographicCitation.doi
10.3390/ijms21062165
dcterms.bibliographicCitation.journaltitle
International Journal of Molecular Sciences
dcterms.bibliographicCitation.originalpublishername
MDPI AG
dcterms.bibliographicCitation.volume
21
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
32245213
dcterms.isPartOf.eissn
1422-0067