dc.contributor.author
Sandner, Georg
dc.contributor.author
Mueller, Andreas S.
dc.contributor.author
Zhou, Xiaodan
dc.contributor.author
Stadlbauer, Verena
dc.contributor.author
Schwarzinger, Bettina
dc.contributor.author
Schwarzinger, Clemens
dc.contributor.author
Wenzel, Uwe
dc.contributor.author
Maenner, Klaus
dc.contributor.author
Klis, Jan Dirk van der
dc.contributor.author
Hirtenlehner, Stefan
dc.contributor.author
Aumiller, Tobias
dc.contributor.author
Weghuber, Julian
dc.date.accessioned
2020-06-22T09:28:43Z
dc.date.available
2020-06-22T09:28:43Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/27683
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-27437
dc.description.abstract
Climatic changes and heat stress have become a great challenge in the livestock industry, negatively affecting, in particular, poultry feed intake and intestinal barrier malfunction. Recently, phytogenic feed additives were applied to reduce heat stress effects on animal farming. Here, we investigated the effects of ginseng extract using various in vitro and in vivo experiments. Quantitative real-time PCR, transepithelial electrical resistance measurements and survival assays under heat stress conditions were carried out in various model systems, including Caco-2 cells, Caenorhabditis elegans and jejunum samples of broilers. Under heat stress conditions, ginseng treatment lowered the expression of HSPA1A (Caco-2) and the heat shock protein genes hsp-1 and hsp-16.2 (both in C. elegans), while all three of the tested genes encoding tight junction proteins, CLDN3, OCLN and CLDN1 (Caco-2), were upregulated. In addition, we observed prolonged survival under heat stress in Caenorhabditis elegans, and a better performance of growing ginseng-fed broilers by the increased gene expression of selected heat shock and tight junction proteins. The presence of ginseng extract resulted in a reduced decrease in transepithelial resistance under heat shock conditions. Finally, LC-MS analysis was performed to quantitate the most prominent ginsenosides in the extract used for this study, being Re, Rg1, Rc, Rb2 and Rd. In conclusion, ginseng extract was found to be a suitable feed additive in animal nutrition to reduce the negative physiological effects caused by heat stress. View Full-Text
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Ginseng extract
en
dc.subject
intestinal barrier
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
dc.title
Ginseng Extract Ameliorates the Negative Physiological Effects of Heat Stress by Supporting Heat Shock Response and Improving Intestinal Barrier Integrity
dc.type
Wissenschaftlicher Artikel
dc.title.subtitle
Evidence from Studies with Heat-Stressed Caco-2 Cells, C. elegans and Growing Broilers
dcterms.bibliographicCitation.articlenumber
835
dcterms.bibliographicCitation.doi
10.3390/molecules25040835
dcterms.bibliographicCitation.journaltitle
Molecules
dcterms.bibliographicCitation.number
4
dcterms.bibliographicCitation.originalpublishername
MDPI
dcterms.bibliographicCitation.volume
25
dcterms.bibliographicCitation.url
https://doi.org/10.3390/molecules25040835
refubium.affiliation
Veterinärmedizin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1420-3049
dcterms.isPartOf.zdb
2008644-1