dc.contributor.author
Poller, Wolfram C.
dc.contributor.author
Löwa, Norbert
dc.contributor.author
Schleicher, Moritz
dc.contributor.author
Münster-Wandowski, Agnieszka
dc.contributor.author
Taupitz, Matthias
dc.contributor.author
Stangl, Verena
dc.contributor.author
Ludwig, Antje
dc.contributor.author
Wiekhorst, Frank
dc.date.accessioned
2020-04-01T09:53:00Z
dc.date.available
2020-04-01T09:53:00Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/27048
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-26809
dc.description.abstract
Interaction with biological material can alter physicochemical parameters of magnetic nanoparticles and might thereby change their magnetic behavior with potentially important implications for various nanoparticle applications. Little is known about changes of the magnetic behavior that occur during the initial phase of cell binding and uptake. We investigate the magnetic behavior of very small superparamagnetic iron-oxide nanoparticles (VSOP) during initial contact with THP-1 monocytes. We combine real-time magnetic particle spectroscopy (MPS), a fast and sensitive method for specific detection of magnetic nanoparticles in biological specimen with high-pressure-freezing/freeze-substitution transmission electron microscopy (HPF/FS-TEM), enabling us to generate snapshots of the interaction of VSOP with the cellular glycocalyx. MPS reveals significant changes of the dynamic magnetic behavior within seconds after VSOP injection into monocyte suspensions that correlate with the formation of nanoparticle clusters in the glycocalyx. The combination of real-time MPS and HPF/FS-TEM provides an ideal platform to analyze magnetic behaviors of nanoparticles upon interaction with cells and tissues.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
physicochemical parameters
en
dc.subject
magnetic nanoparticles
en
dc.subject
magnetic particle spectroscopy
en
dc.subject
electron microscopy
en
dc.subject
real-time MPS
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Initial interaction of citrate-coated iron oxide nanoparticles with the glycocalyx of THP-1 monocytes assessed by real-time magnetic particle spectroscopy and electron microscopy
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
3591
dcterms.bibliographicCitation.doi
10.1038/s41598-020-60162-8
dcterms.bibliographicCitation.journaltitle
Scientific Reports
dcterms.bibliographicCitation.originalpublishername
Nature Publishing Group
dcterms.bibliographicCitation.volume
10
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
32107402
dcterms.isPartOf.eissn
2045-2322