dc.contributor.author
Xi, De
dc.contributor.author
Alter, Thomas
dc.contributor.author
Einspanier, Ralf
dc.contributor.author
Sharbati, Soroush
dc.contributor.author
Gölz, Greta
dc.date.accessioned
2020-03-24T09:38:58Z
dc.date.available
2020-03-24T09:38:58Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/27006
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-26767
dc.description.abstract
Background
Campylobacter jejuni (C. jejuni) has been assigned as an important food-borne pathogen for human health but many pathogenicity factors of C. jejuni and human host cell responses related to the infection have not yet been adequately clarified. This study aimed to determine further C. jejuni pathogenicity factors and virulence genes based on a random mutagenesis approach. A transposon mutant library of C. jejuni NCTC 11168 was constructed and the ability of individual mutants to adhere to and invade human intestinal epithelial cells was evaluated compared to the wild type. We identified two mutants of C. jejuni possessing altered phenotypes with transposon insertions in the genes Cj1492c and Cj1507c. Cj1492c is annotated as a two-component sensor and Cj1507c is described as a regulatory protein. However, functions of both mutated genes are not clarified so far.
Results
In comparison to the wild type, Cj::1492c and Cj::1507c showed around 70–80% relative motility and Cj::1492c had around 3-times enhanced adhesion and invasion rates whereas Cj::1507c had significantly impaired adhesive and invasive capability. Moreover, Cj::1492c had a longer lag phase and slower growth rate while Cj::1507c showed similar growth compared to the wild type. Between 5 and 24 h post infection, more than 60% of the intracellular wild type C. jejuni were eliminated in HT-29/B6 cells, however, significantly fewer mutants were able to survive intracellularly. Nevertheless, no difference in host cell viability and induction of the pro-inflammatory chemokine IL-8 were determined between both mutants and the wild type.
Conclusion
We conclude that genes regulated by Cj1507c have an impact on efficient adhesion, invasion and intracellular survival of C. jejuni in HT-29/B6 cells. Furthermore, potential signal sensing by Cj1492c seems to lead to limiting attachment and hence internalisation of C. jejuni. However, as the intracellular survival capacities are reduced, we suggest that signal sensing by Cj1492c impacts several processes related to pathogenicity of C. jejuni.
en
dc.format.extent
11 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Campylobacter jejuni
en
dc.subject
pathogenicity
en
dc.subject
host cell response
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Campylobacter jejuni genes Cj1492c and Cj1507c are involved in host cell adhesion and invasion
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
8
dcterms.bibliographicCitation.doi
10.1186/s13099-020-00347-8
dcterms.bibliographicCitation.journaltitle
Gut pathogens
dcterms.bibliographicCitation.volume
12
dcterms.bibliographicCitation.url
https://doi.org/10.1186/s13099-020-00347-8
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Lebensmittelsicherheit und -hygiene
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
1757-4749
refubium.resourceType.provider
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