dc.contributor.author
Chen, Shuai
dc.contributor.author
Einspanier, Ralf
dc.contributor.author
Schoen, Jennifer
dc.date.accessioned
2020-02-28T11:10:08Z
dc.date.available
2020-02-28T11:10:08Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/26783
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-26540
dc.description.abstract
Cultivation of oviduct epithelial cells on porous filters fosters in vivo-like morphology and functionality. However, due to the optical properties of the filter materials and the cells’ columnar shape, cell quality is hard to assess via light microscopy. In this study, we aim to evaluate transepithelial electrical resistance (TEER) measurement as a prognostic quality indicator for the cultivation of porcine oviduct epithelial cells (POEC). POEC were maintained in four different types of media for 3 and 6 w to achieve diverse culture qualities, and TEER was measured before processing samples for histology. Culture quality was scored using morphological criteria (presence of cilia, confluence and cell polarity). We furthermore analyzed the correlation between cellular height (as a measure of apical–basal polarization) and TEER in fully differentiated routine cultures (biological variation) and in cultures with altered cellular height due to hormonal stimulation. Fully differentiated cultures possessed a moderate TEER between 500 and 1100 Ω*cm2. Only 5 % of cultures which exhibited TEER values in this defined range had poor quality. Sub-differentiated cultures showed either very low or excessively high TEER. We unveiled a highly significant (P < 0.0001) negative linear correlation between TEER and epithelial height in well-differentiated cultures (both routine and hormone stimulated group). This may point toward the interaction between tight junction assembly and epithelial apical–basal polarization. In conclusion, TEER is a straightforward quality indicator which could be routinely used to monitor the differentiation status of oviduct epithelial cells in vitro.
en
dc.format.extent
7 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
oviduct epithelial cells
en
dc.subject
culture quality
en
dc.subject
tight junctions
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::590 Tiere (Zoologie)::590 Tiere (Zoologie)
dc.subject.ddc
500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::572 Biochemie
dc.title
Transepithelial electrical resistance (TEER): a functional parameter to monitor the quality of oviduct epithelial cells cultured on filter supports
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.doi
10.1007/s00418-015-1351-1
dcterms.bibliographicCitation.journaltitle
Histochemistry and cell biology
dcterms.bibliographicCitation.pagestart
509
dcterms.bibliographicCitation.pageend
515
dcterms.bibliographicCitation.volume
144
dcterms.bibliographicCitation.url
https://doi.org/10.1007/s00418-015-1351-1
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Veterinär-Biochemie
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.issn
0948-6143
dcterms.isPartOf.eissn
1432-119X