dc.contributor.author
Haase, Andrea
dc.contributor.author
Rott, Stephanie
dc.contributor.author
Mantion, Alexandre
dc.contributor.author
Graf, Philipp
dc.contributor.author
Plendl, Johanna
dc.contributor.author
Thünemann, Andreas F.
dc.contributor.author
Meier, Wolfgang P.
dc.contributor.author
Taubert, Andreas
dc.contributor.author
Luch, Andreas
dc.contributor.author
Reiser, Georg
dc.date.accessioned
2020-02-26T10:45:58Z
dc.date.available
2020-02-26T10:45:58Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/26752
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-26509
dc.description.abstract
In the body, nanoparticles can be systemically distributed and then may affect secondary target organs, such as the central nervous system (CNS). Putative adverse effects on the CNS are rarely investigated to date. Here, we used a mixed primary cell model consisting mainly of neurons and astrocytes and a minor proportion of oligodendrocytes to analyze the effects of well-characterized 20 and 40 nm silver nanoparticles (SNP). Similar gold nanoparticles served as control and proved inert for all endpoints tested. SNP induced a strong size-dependent cytotoxicity. Additionally, in the low concentration range (up to 10 μg/ml of SNP), the further differentiated cultures were more sensitive to SNP treatment. For detailed studies, we used low/medium dose concentrations (up to 20 μg/ml) and found strong oxidative stress responses. Reactive oxygen species (ROS) were detected along with the formation of protein carbonyls and the induction of heme oxygenase-1. We observed an acute calcium response, which clearly preceded oxidative stress responses. ROS formation was reduced by antioxidants, whereas the calcium response could not be alleviated by antioxidants. Finally, we looked into the responses of neurons and astrocytes separately. Astrocytes were much more vulnerable to SNP treatment compared with neurons. Consistently, SNP were mainly taken up by astrocytes and not by neurons. Immunofluorescence studies of mixed cell cultures indicated stronger effects on astrocyte morphology. Altogether, we can demonstrate strong effects of SNP associated with calcium dysregulation and ROS formation in primary neural cells, which were detectable already at moderate dosages.
en
dc.format.extent
12 Seiten
dc.rights.uri
https://creativecommons.org/licenses/by-nc/4.0/
dc.subject
silver nanoparticles
en
dc.subject
oxidative stress
en
dc.subject
protein carbonyls
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Effects of silver nanoparticles on primary mixed neural cell cultures: Uptake, oxidative stress and acute calcium responses
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.doi
10.1093/toxsci/kfs003
dcterms.bibliographicCitation.journaltitle
Toxicological sciences
dcterms.bibliographicCitation.number
2
dcterms.bibliographicCitation.pagestart
457
dcterms.bibliographicCitation.pageend
468
dcterms.bibliographicCitation.volume
126
dcterms.bibliographicCitation.url
https://doi.org/10.1093/toxsci/kfs003
refubium.affiliation
Veterinärmedizin
refubium.affiliation.other
Institut für Veterinär-Anatomie
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refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.issn
1096-6080
dcterms.isPartOf.eissn
1096-0929