dc.contributor.author
Rancan, Fiorenza
dc.contributor.author
Contardi, Marco
dc.contributor.author
Jurisch, Jana
dc.contributor.author
Blume-Peytavi, Ulrike
dc.contributor.author
Vogt, Annika
dc.contributor.author
Bayer, Ilker S.
dc.contributor.author
Schaudinn, Christoph
dc.date.accessioned
2020-01-20T14:36:16Z
dc.date.available
2020-01-20T14:36:16Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/26464
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-26224
dc.description.abstract
Topical treatment of wound infections is often a challenge due to limited drug availability at the site of infection. Topical drug delivery is an attractive option for reducing systemic side effects, provided that a more selective and sustained local drug delivery is achieved. In this study, a poorly water-soluble antibiotic, ciprofloxacin, was loaded on polyvinylpyrrolidone (PVP)-based foils and nanofiber mats using acetic acid as a solubilizer. Drug delivery kinetics, local toxicity, and antimicrobial activity were tested on an ex vivo wound model based on full-thickness human skin. Wounds of 5 mm in diameter were created on 1.5 × 1.5 cm skin blocks and treated with the investigated materials. While nanofiber mats reached the highest amount of delivered drug after 6 h, foils rapidly achieved a maximum drug concentration and maintained it over 24 h. The treatment had no effect on the overall skin metabolic activity but influenced the wound healing process, as observed using histological analysis. Both delivery systems were efficient in preventing the growth of Pseudomonas aeruginosa biofilms in ex vivo human skin. Interestingly, foils loaded with 500 µg of ciprofloxacin accomplished the complete eradication of biofilm infections with 1 × 109 bacteria/wound. We conclude that antimicrobial-loaded resorbable PVP foils and nanofiber mats are promising delivery systems for the prevention or topical treatment of infected wounds.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
wound infection
en
dc.subject
pseudomonas aeruginosa
en
dc.subject
antimicrobial delivery
en
dc.subject
polyvinylpyrrolidone
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Evaluation of Drug Delivery and Efficacy of Ciprofloxacin-Loaded Povidone Foils and Nanofiber Mats in a Wound-Infection Model Based on Ex Vivo Human Skin
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
527
dcterms.bibliographicCitation.doi
10.3390/pharmaceutics11100527
dcterms.bibliographicCitation.journaltitle
Pharmaceutics
dcterms.bibliographicCitation.number
10
dcterms.bibliographicCitation.originalpublishername
MDPI AG
dcterms.bibliographicCitation.volume
11
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
31614886
dcterms.isPartOf.eisbn
1999-4923