dc.contributor.author
Hoffmann, Maximilian
dc.contributor.author
Judkewitz, Benjamin
dc.date.accessioned
2020-01-13T12:59:37Z
dc.date.available
2020-01-13T12:59:37Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/26385
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-26145
dc.description.abstract
Imaging of neuronal activity with fluorescent indicators is an important technique in neuroscience. However, it remains challenging to record volumetric image data at fast frame rates and good resolution. One promising technique to achieve this goal is light sheet microscopy (LSM), but the right angle configuration of the excitation and imaging system limits its application. Oblique plane microscopy (OPM), a variant of LSM, circumvents this limitation by exciting oblique planes and detecting the image through the same microscope objective lens. So far, these techniques have relied on the use of high numerical aperture (NA) detection objective lenses, which limits their field of view. Here we present an OPM technique that allows for the use of low NA objective lenses by redirecting the light with the help of a diffraction grating. The microscope maintains a micrometer-scale lateral resolution over a large addressable imaging volume of 3.3×3.0×1.0 mm3. We demonstrate its practicality by imaging the whole brain of larval and juvenile zebrafish.
en
dc.subject.ddc
500 Natural sciences and mathematics::530 Physics::530 Physics
dc.subject.ddc
500 Natural sciences and mathematics::570 Life sciences::570 Life sciences
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Diffractive oblique plane microscopy
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.doi
10.1364/OPTICA.6.001166
dcterms.bibliographicCitation.journaltitle
Optica
dcterms.bibliographicCitation.number
9
dcterms.bibliographicCitation.pagestart
1166
dcterms.bibliographicCitation.pageend
1170
dcterms.bibliographicCitation.volume
6
dcterms.rightsHolder.note
© 2019 Optical Society of America. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.
dcterms.rightsHolder.url
https://www.osapublishing.org/library/license_v1.cfm
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.isSupplementedBy.doi
10.6084/m9.figshare.8938253
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
2334-2536