dc.contributor.author
Ostendorf, Lennard
dc.contributor.author
Mothes, Ronja
dc.contributor.author
Koppen, Sofie van
dc.contributor.author
Lindquist, Randall L.
dc.contributor.author
Bellmann-Strobl, Judith
dc.contributor.author
Asseyer, Susanna
dc.contributor.author
Ruprecht, Klemens
dc.contributor.author
Alexander, Tobias
dc.contributor.author
Niesner, Raluca A.
dc.contributor.author
Hauser, Anja E.
dc.contributor.author
Paul, Friedemann
dc.contributor.author
Radbruch, Helena
dc.date.accessioned
2020-01-09T11:52:37Z
dc.date.available
2020-01-09T11:52:37Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/26360
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-26121
dc.description.abstract
Objective: To investigate whether low-density granulocytes (LDGs) are an immunophenotypic feature of patients with multiple sclerosis (MS) or neuromyelitis optica spectrum disorder (NMOSD).
Methods: Blood samples were collected from 20 patients with NMOSD and 17 patients with MS, as well as from 15 patients with Systemic Lupus Erythematosus (SLE) and 23 Healthy Donors (HD). We isolated peripheral blood mononuclear cells (PBMCs) with density gradient separation and stained the cells with antibodies against CD14, CD15, CD16, and CD45, and analyzed the cells by flow cytometry or imaging flow cytometry. We defined LDGs as CD14-CD15(high) and calculated their share in total PBMC leukocytes (CD45+) as well as the share of CD16(hi) LDGs. Clinical data on disease course, medication, and antibody status were obtained.
Results: LDGs were significantly more common in MS and NMOSD than in HDs, comparable to SLE samples (median values HD 0.2%, MS 0.9%, NMOSD 2.1%, SLE 4.3%). 0/23 of the HDs, but 17/20 NMOSD and 11/17 MS samples as well as 13/15 SLE samples had at least 0.7 % LDGs. NMOSD patients without continuous immunosuppressive treatment had significantly more LDGs compared to their treated counterparts. LDG nuclear morphology ranged from segmented to rounded, suggesting a heterogeneity within the group.
Conclusion: LDGs are a feature of the immunophenotype in some patients with MS and NMOSD.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
low-density granulocytes
en
dc.subject
multiple sclerosis
en
dc.subject
neuromyelitis optica
en
dc.subject
neuroinflammation
en
dc.subject
autoimmunity
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Low-Density Granulocytes Are a Novel Immunopathological Feature in Both Multiple Sclerosis and Neuromyelitis Optica Spectrum Disorder
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
2725
dcterms.bibliographicCitation.doi
10.3389/fimmu.2019.02725
dcterms.bibliographicCitation.journaltitle
Frontiers in Immunology
dcterms.bibliographicCitation.originalpublishername
Frontiers Media S.A.
dcterms.bibliographicCitation.volume
10
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
31849944
dcterms.isPartOf.eissn
1664-3224