dc.contributor.author
Luo, Ying
dc.contributor.author
Meyer, Nicole
dc.contributor.author
Jiao, Qingqing
dc.contributor.author
Scheffel, Jörg
dc.contributor.author
Zimmermann, Carolin
dc.contributor.author
Metz, Martin
dc.contributor.author
Zenclussen, Ana
dc.contributor.author
Maurer, Marcus
dc.contributor.author
Siebenhaar, Frank
dc.date.accessioned
2019-12-13T09:23:44Z
dc.date.available
2019-12-13T09:23:44Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/26217
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-25977
dc.description.abstract
Mast cells (MCs) are considered as key effector cells in the elicitation of allergic symptoms, and they are essential players in innate and adaptive immune responses. In mice, two main types of MCs have been described: connective tissue MCs (CTMCs) and mucosal MCs (MMCs). However, little is known about the biological functions of MMCs, which is due to the lack of suitable models to investigate MMCs in vivo. Here, we aimed to generate a mouse model selectively deficient in MMCs. It has been previously described that Cre expressed under the control of the baboon α-chymase promotor is predominantly localized in MMCs. Therefore, we mated α-chymase-Cre transgenic mice with mice bearing a floxed allele of the myeloid cell leukemia sequence 1 (Mcl-1). Mcl-1 encodes for an intracellular antiapoptotic factor in MCs; hence, a selective reduction in MMCs was expected. Our results show that this new mouse model contains markedly reduced numbers of MMCs in mucosal tissues, whereas numbers of CTMCs are normal. Thus, Chm-Cre; Mcl-1fl/fl mice are a useful tool for the investigation of the pathophysiological functions of MMCs in vivo.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
connective tissue
en
dc.subject.ddc
600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit
dc.title
Chymase-Cre; Mcl-1fl/fl Mice Exhibit Reduced Numbers of Mucosal Mast Cells
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.articlenumber
2399
dcterms.bibliographicCitation.doi
10.3389/fimmu.2019.02399
dcterms.bibliographicCitation.journaltitle
Frontiers in Immunology
dcterms.bibliographicCitation.originalpublishername
Frontiers Media S.A.
dcterms.bibliographicCitation.volume
10
refubium.affiliation
Charité - Universitätsmedizin Berlin
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.bibliographicCitation.pmid
31681290
dcterms.isPartOf.eissn
1664-3224