dc.contributor.author
Alagesan, Kathirvel
dc.contributor.author
Kolarich, Daniel
dc.date.accessioned
2019-12-10T12:11:30Z
dc.date.available
2019-12-10T12:11:30Z
dc.identifier.uri
https://refubium.fu-berlin.de/handle/fub188/26078
dc.identifier.uri
http://dx.doi.org/10.17169/refubium-25837
dc.description.abstract
Chicken egg yolk is an easily available source for the isolation of sialylglycopeptides (SGP) carrying homogenous biantennary N-glycans. This approach has gained much attention in the last decade since these SGPs can easily be used for the semi-synthesis of glycoconjugates circumventing laborious full-synthetic methodologies. Here we report an optimised, significantly shorter (one day instead of five) and environmentally friendly procedure for the mg scale isolation of SGP using commercially available egg yolk powder. A single chromatographic step following chloroform/methanol precipitation of proteins and lipids yielded desired approximately 200 mg SGP from 250 g egg yolk powder within a day.
•Environmentally friendly procedure for isolation of sialylglycopeptide from Egg yolk powder.
•Reduced the protocol from five days down to one.
en
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
dc.subject
Synthetic glycobiology
en
dc.subject
Glycopeptide isolation
en
dc.subject
Egg yolk powder
en
dc.subject.ddc
500 Naturwissenschaften und Mathematik::540 Chemie::540 Chemie und zugeordnete Wissenschaften
dc.title
Improved strategy for large scale isolation of sialylglycopeptide (SGP) from egg yolk powder
dc.type
Wissenschaftlicher Artikel
dcterms.bibliographicCitation.doi
10.1016/j.mex.2019.04.007
dcterms.bibliographicCitation.journaltitle
MethodsX
dcterms.bibliographicCitation.pagestart
773
dcterms.bibliographicCitation.pageend
778
dcterms.bibliographicCitation.volume
6
dcterms.bibliographicCitation.url
https://doi.org/10.1016/j.mex.2019.04.007
refubium.affiliation
Biologie, Chemie, Pharmazie
refubium.affiliation.other
Institut für Chemie und Biochemie
refubium.resourceType.isindependentpub
no
dcterms.accessRights.openaire
open access
dcterms.isPartOf.eissn
2215-0161
